Citas bibligráficas
Mendoza, R., (2017). Hidrólisis química y enzimática de un extracto de yacón (Smallanthus sonchifolius) para la obtención de fructosa [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/2663
Mendoza, R., Hidrólisis química y enzimática de un extracto de yacón (Smallanthus sonchifolius) para la obtención de fructosa [Tesis]. : Universidad Nacional Agraria La Molina; 2017. https://hdl.handle.net/20.500.12996/2663
@misc{renati/1116154,
title = "Hidrólisis química y enzimática de un extracto de yacón (Smallanthus sonchifolius) para la obtención de fructosa",
author = "Mendoza Sandoval, Rocío Milagros",
publisher = "Universidad Nacional Agraria La Molina",
year = "2017"
}
The maximum conversion parameters of sugars: sucrose and fructooligosaccharides (FOS) from a yacon extract to fructose were optimized using acid and enzymatic hydrolysis. For the acid hydrolysis, sulfuric and phosphoric acids were used independently and, for the enzyme, inulinase and invertase were used, independently and mixed. A Box-Behnken design was used to optimize the yield of fructose in acid hydrolysis. The surface response models were adjusted successfully in both acids (R2 = 0.963 - 0.938). The optimum conditions in the hydrolysis with sulfuric acid were pH 1, temperature 88.4 °C and 38.7 min getting 9.118 g fructose/liter extract of yield; while with phosphoric acid the conditions were: pH 1, temperature 89.9 °C and 42.2 min getting 7.848 g fructose/liter extract yield. For enzymatic hydrolysis a Central Composite design was employed, the response surface models were also successfully adjusted in both, for inulinase better than for invertase (R2 =0.983 - 0.880). The optimum conditions of the enzymatic hydrolysis using inulinase were 134 min and 11.7 U/g FOS; while with the invertase were: time of 127.5 min and 0.674 U/g FOS, both reactions under the constant conditions of 50 °C and pH 5, getting 6.168 and 7.506 g fructose/liter of extract yield, respectively. Finally, with the Mixtures design, the optimum amounts of concentration of both enzymes and percentage of FOS were: 1.930 x 10-10 U inulinase /g FOS, 0.048 U invertase /g FOS and 4.55 percent FOS getting 56.31 g fructose / liter extract yield; the constant factors were: 50 °C, pH 5 and 2 hours; with a R2 =0.999.
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons