Citas bibligráficas
Pesantes, B., De, M. (2023). Caracterización bioinformática de aptámeros de ADN seleccionados contra la proteína quinasa (PknG) de Mycobacterium tuberculosis [Tesis, Universidad Peruana de Ciencias Aplicadas (UPC)]. http://hdl.handle.net/10757/671053
Pesantes, B., De, M. Caracterización bioinformática de aptámeros de ADN seleccionados contra la proteína quinasa (PknG) de Mycobacterium tuberculosis [Tesis]. PE: Universidad Peruana de Ciencias Aplicadas (UPC); 2023. http://hdl.handle.net/10757/671053
@misc{sunedu/4393032,
title = "Caracterización bioinformática de aptámeros de ADN seleccionados contra la proteína quinasa (PknG) de Mycobacterium tuberculosis",
author = "De Zavala Romaña, Maria Paz",
publisher = "Universidad Peruana de Ciencias Aplicadas (UPC)",
year = "2023"
}
The PknG enzyme is one of 11 serine-threonine kinases present in Mycobacterium tuberculosis. These enzymes regulate different physiological processes. It has been reported that PknG is responsible for inhibiting lysosome/phagosome binding within macrophages and thus ensuring the survival of the bacterium. During the last decade it has been proposed that inhibition of PknG may have the potential to affect the disease in its latency period (3). In this study we bioinformatically analyzed DNA aptamers against PknG and its kinase (RK) domain from 7 enriched libraries. The Fast Aptamer program was used to perform selection filters based on number of reads, RPM, and abundance. Additionally, the Mfold and RNA Composer programs were used to visualize possible secondary structures. During the analysis, an average of 41104 sequences were observed among all the libraries. When analyzing the enrichment ratios, it was observed that only 10% of the sequences against the whole protein and 8.9% of the sequences against the RK domain had an enrichment ratio greater than two. From the five libraries selected against the target protein, a TOP10 was selected based on the abundance of the sequences. It was observed that the highest average enrichment ratio (3.68) was found in TOP10 of the library subjected to a PknG concentration of 20 nM. Finally, from the TOP10 of the five libraries, nine aptamers were selected according to the frequency of occurrence in more than one library.
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