Citas bibligráficas
Pacheco, E., (2015). Caracterización morfológica y molecular de mashua (Tropaeolum tuberosum Ruiz y Pavón) de los departamentos de Cusco y Cajamarca [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/2095
Pacheco, E., Caracterización morfológica y molecular de mashua (Tropaeolum tuberosum Ruiz y Pavón) de los departamentos de Cusco y Cajamarca [Tesis]. : Universidad Nacional Agraria La Molina; 2015. https://hdl.handle.net/20.500.12996/2095
@mastersthesis{sunedu/3951568,
title = "Caracterización morfológica y molecular de mashua (Tropaeolum tuberosum Ruiz y Pavón) de los departamentos de Cusco y Cajamarca",
author = "Pacheco Arenas, Erika Samamtha",
publisher = "Universidad Nacional Agraria La Molina",
year = "2015"
}
Mashua (Tropaeolum tuberosum Ruiz & Pavón) is a vegetatively propagated Andean tuber, with great agronomic, nutritional and phytochemical potential. However, there are very few studies of diversity on this crop, due to the lack of market demand and bad postharvest management. Therefore, this work aims to contribute to the morphological and molecular variability knowledge of mashua landraces from Cusco and Cajamarca o Regions. Morphological characterizations were performed on 271 mashua accessions using 45 descriptors previously established. The identification of more discriminating and informative descriptors was performed by sequential multiple correspondence analysis (ACMs). Then, cluster analysis and identification of morphotypes were made with the new list of descriptors. The molecular characterization was made using six AFLP (Amplified fragment length polymorphism) primer combinations and, with the recorded polymorphisms were performed clusters analysis using the simple matching (SM) coefficient and principal coordinate analysis. In addition, population differentiation analysis was made using Analysis of molecular variance (AMOVA) and allelic fixation techniques (Fst). Nineteen morphological descriptors were selected for mashua, which allowed to identify 257 morphotypes (237 from Cusco and 20 from Cajamarca) according to 0 Euclidean coefficients. Moreover, molecular characterization did not show any duplicates at 1 SM coefficient; however, allowed the separation on two groups according to their geographical origin of the accessions. Likewise, both the AMOVA and allelic fixation (FST = 0.1980) showed genetic differentiation between both subpopulations. Further, there is not congruence between morphological descriptors and molecular markers (low correlation r = -0.32093); which suggest that for an accurate characterization of diversity both techniques are necessary. These results show a high variability in this crop, which will help to plan future strategies on germoplasm management and improvement of mashua in Peru.
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