Bibliographic citations
Arbaiza, M., (2020). Efecto de la criopreservación en fragmentación del ADN, viabilidad, motilidad y cinética espermática en toros Brown Swiss empleando sistema casa [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4297
Arbaiza, M., Efecto de la criopreservación en fragmentación del ADN, viabilidad, motilidad y cinética espermática en toros Brown Swiss empleando sistema casa []. PE: Universidad Nacional Agraria La Molina; 2020. https://hdl.handle.net/20.500.12996/4297
@mastersthesis{sunedu/3286821,
title = "Efecto de la criopreservación en fragmentación del ADN, viabilidad, motilidad y cinética espermática en toros Brown Swiss empleando sistema casa",
author = "Arbaiza Barnechea, Martin Daniel",
publisher = "Universidad Nacional Agraria La Molina",
year = "2020"
}
The objective of this research was to evaluate the effect of sperm cryopreservation on DNA fragmentation, viability and sperm kinetics using the computer-assisted semen analysis (CASA), 5 bulls of the Brown Swiss breed located in the National Semen Bank of the UNALM were collected, we proceeded to collect, process and freeze the 76 ejaculates obtained. For the analysis of DNA fragmentation the Sperm-Halomax® Kit was used and for the analysis of the sperm viability Hoeschst 333242 / PI stain, it was evaluated if there was a significant change between the values before and after the cryopreservation for each one of the seminal parameters evaluated using the T test of related samples, after verification of normality with the Shapiro-Wilk test in case the data were not normal, the Wilcoxon signed rank test was used. The Bonferroni adjustment was applied for the referred above comparisons. All the analyzes were made considering 5 biological replicas in the statistical package SPSS v.23 with a 95% reliability. Significant differences were found in 16 of the 21 parameters evaluated, an increase in DNA fragmentation of 3% was evidenced, in the sperm viability parameter a decrease in the analyzed values was manifested. The decrease in the values of the following parameters motility, progressive motility, rapid motility was observed, as were the kinetic parameters VCL, VSL, VAP, DSL, DAP, ALH, BCF, HAC, STR, and an increase in the values of local motility and immobile sperm, attributed to the effect of seminal cryopreservation.
This item is licensed under a Creative Commons License