Citas bibligráficas
De, Y., (2020). Efecto de citoquininas en el cultivo in vitro de dos especies de Berries nativos del Perú: Vaccinium floribundum Kunth <<pushgay>> y Macleanía rupestris Kunth A. C. Smith <<alicon>> [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4385
De, Y., Efecto de citoquininas en el cultivo in vitro de dos especies de Berries nativos del Perú: Vaccinium floribundum Kunth <<pushgay>> y Macleanía rupestris Kunth A. C. Smith <<alicon>> []. PE: Universidad Nacional Agraria La Molina; 2020. https://hdl.handle.net/20.500.12996/4385
@misc{sunedu/3161785,
title = "Efecto de citoquininas en el cultivo in vitro de dos especies de Berries nativos del Perú: Vaccinium floribundum Kunth <<pushgay>> y Macleanía rupestris Kunth A. C. Smith <<alicon>>",
author = "De la Cruz Chacon, Yngrid",
publisher = "Universidad Nacional Agraria La Molina",
year = "2020"
}
Peruvian native berries have a high nutritional value; however, only few of them have been domesticated. Their fruits have different sizes, quality and flavor that make these species a very important genetic bank for breeding programs. The objective of this research is to assess the in vitro propagation of two species of Peruvian native berries, namely, Vaccinium floribundum Kunth and Macleania rupestris Kunth A. C. Smith -known as “pushgay” and “alicon”, respectively- using different culture media, supplemented with different cytokinin concentrations (zeatin and kinetin). The basal culture medium used was Lloyd and McCown´s medium (1980), known as Woody Plant (WP), and, as growth regulators, stock solutions of zeatin 1000 ppm or kinetin 1000 ppm. Work was carried out in a controlled environment, with a temperature of 22 ºC +/- 2 ºC, photoperiod of 8/16 hours and 47.74 μmol.m-2.s-1 luminosity. The experimental design used was a completely random (DCA), involving four treatments and seven repetitions, during a period of 120 days. The response variables were length of the explant, number of leaves, number of shoots and number of roots. The data were analyzed by a simple variance analysis (ANOVA) and Tukey test, where significant differences were obtained in each of the variables. It was observed that the treatments supplement with kinetin were better, resulting in explants with longer length, longer internodes, greener leaves and root presence, whereas in the treatment with zeatin, only calluses were observed. It was concluded that the propagation medium for “pushgay” is the one supplemented with 1.0 ppm kinetin and, for “alicon” the medium supplemented with 0.5 ppm kinetin.
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