Citas bibligráficas
Morón, J., (2016). Verificación de la genealogía en alpacas (Vicugna pacos) usando un panel de microsatélites [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/2706
Morón, J., Verificación de la genealogía en alpacas (Vicugna pacos) usando un panel de microsatélites [Tesis]. : Universidad Nacional Agraria La Molina; 2016. https://hdl.handle.net/20.500.12996/2706
@mastersthesis{sunedu/3014246,
title = "Verificación de la genealogía en alpacas (Vicugna pacos) usando un panel de microsatélites",
author = "Morón Barraza, Jonathan Alejandro",
publisher = "Universidad Nacional Agraria La Molina",
year = "2016"
}
The aim was to verify the genealogy information in Huacaya alpaca using microsatellite markers. The specific objectives were: a) to validate a panel of microsatellite markers for paternity testing; b) to estimate the inbreeding level by production unit and; c) to assign paternity for cases in the presence or absence of parents. 15 microsatellite markers for alpaca were used. DNA was extracted from hair follicles of 247 huacaya alpaca belonging to three production units in Pasco Region. The results obtained were: a) the total number of alleles ranged from 8 to 32 alleles per locus, the polymorphic information content was greater than 0.7 in all markers used in alpacas. The estimated values of the probability of discrimination for markers LCA37, LCA66, LCA08, YWLL36, YWLL44, YWLL08, VOLP04, and VOLP32 were greater than 0.84, and the probability of exclusion joint was higher than 99.9%. b) The FIS value was 0.232, which indicates a high level of inbreeding. c) The allocation of paternity had a 100% success. In conclusion, 77 out of 116 cases of paternity verification matched with the field records of the three production units. The use of the panel of microsatellite markers was convenient and efficient for individual identification and parentage testing in huacaya alpacas.
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