Citas bibligráficas
Suárez, M., (2014). Efecto de la suplementación de L-cisteína en el dilutor Tes-Tris-Yema, sobre la calidad espermática post-descongelamiento en alpaca (Vicugna pacos) [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/3768
Suárez, M., Efecto de la suplementación de L-cisteína en el dilutor Tes-Tris-Yema, sobre la calidad espermática post-descongelamiento en alpaca (Vicugna pacos) [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2014. https://hdl.handle.net/20.500.12672/3768
@misc{sunedu/2872653,
title = "Efecto de la suplementación de L-cisteína en el dilutor Tes-Tris-Yema, sobre la calidad espermática post-descongelamiento en alpaca (Vicugna pacos)",
author = "Suárez Pinedo, Marilyn Reyna",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2014"
}
The present study evaluated the effect of L-cysteine supplementation to tes-tris-yolk extender on the post-thaw quality of Vicugna pacos “alpaca” sperm. For this purpose, sixteen epididymal sperm samples were collected from male alpaca in the Camal Municipal of Huancavelica - Perú, in a period of four weeks (three replicates). Each sample was diluted in tes-tris-yolk extender and divided into four aliquots of different concentrations of antioxidant: 0 mM L-cysteine (Control), 2.5 mM L-cysteine (Treatment 1), 5 mM L-cysteine (Treatment 2) and 10 mM L-cysteine (Treatment 3). Then the sperm suspensions were loaded in straws (0.25 mL) and were cryopreserved using the slow freezing methodology in a controlled-rate freezer. Finally the straws were stored in the liquid nitrogen. Thawing was performed, at 50°C for 7 s and the sperm motility, viability, plasma membrane and acrosomal integrity were assessed post-thaw. The results of this search show that the supplementation of L-cysteine 2.5 mM to tes-tris-yolk extender significantly improved the motility and plasma membrane integrity compared to the other treatments and the control group (P < 0.05). It is concluded that the addition of L-cysteine (2.5 mM) in the extender gave a cryoprotective effect on the alpaca sperm parameters post-thaw.
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