Citas bibligráficas
Benites, D., (2021). Diagnóstico serológico de toxoplasmosis aguda mediante nuevas combinaciones de antígenos recombinantes quiméricos [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/9270
Benites, D., Diagnóstico serológico de toxoplasmosis aguda mediante nuevas combinaciones de antígenos recombinantes quiméricos []. PE: Universidad Peruana Cayetano Heredia; 2021. https://hdl.handle.net/20.500.12866/9270
@misc{renati/909819,
title = "Diagnóstico serológico de toxoplasmosis aguda mediante nuevas combinaciones de antígenos recombinantes quiméricos",
author = "Benites Tan, Diego Alonso",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2021"
}
Toxoplasmosis, caused by Toxoplasma gondii (TG), is one of the most common zoonosis in the world. Acute infection is asymptomatic, but in immunodeficient people or cases of congenital transmission, it can be mortal. These cases require an early and accurate diagnosis of the acute phase. Serological tests identify antibodies that react with TG antigens. Traditionally, toxoplasma lysate antigen (TLA) is used. Nevertheless, it is inefficient to identify the acute phase. With the use of recombinant antigens (AR) from TG, there has been potential to differentiate phases but with low sensitivity. Combining individual ARs increases sensitivity, but the ability to identify the acute phase is lost. Recombinant chimeric antigens (ARQ) are combinations of AR epitopes. Its usage is easily standardized and gives high sensitivity. The ARQ AMA1-SAG2-GRA1-ROP1 can differentiate phases in IgG avidity tests. But the presence of AMA1 in this ARQ can cause cross-reactions with parasites from the Apicomplexa phylum. In Iquitos, cross-reactions with the genus Plasmodium can occur due to its prevalence in patients with HIV, vulnerable to toxoplasmosis. Thus, it is best to replace AMA1 with an AR that has characterized epitopes, is highly immunogenic, is representative of the acute phase, has no homology with antigens from parasites that infect humans and has a reported high sensitivity; GRA8 has all of these traits. The objective of this work is to evaluate a new ARQ capable of identifying the acute phase of toxoplasmosis and that gives no cross-reactions with antigens from the Plasmodium genus.
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