Bibliographic citations
Guerrero, P., (2018). Estudio fitoquímico del aceite y del extracto metanólico hidrofílico de las semillas de Lúcuma (Pouteria lucuma) procedentes de Chilca, Cañete [Tesis, Pontificia Universidad Católica del Perú]. http://hdl.handle.net/20.500.12404/12137
Guerrero, P., Estudio fitoquímico del aceite y del extracto metanólico hidrofílico de las semillas de Lúcuma (Pouteria lucuma) procedentes de Chilca, Cañete [Tesis]. PE: Pontificia Universidad Católica del Perú; 2018. http://hdl.handle.net/20.500.12404/12137
@mastersthesis{renati/535266,
title = "Estudio fitoquímico del aceite y del extracto metanólico hidrofílico de las semillas de Lúcuma (Pouteria lucuma) procedentes de Chilca, Cañete",
author = "Guerrero Castillo, Pedro Alonso",
publisher = "Pontificia Universidad Católica del Perú",
year = "2018"
}
The aim of this work is to determine the composition of the oil and hydrophilic methanolic extract of lucuma seeds (Pouteria lucuma), which are generated as agroindustrial waste. These come from the district of Chilca (located in the province of Cañete, 62 km from Lima), donated by the OVNI ice cream shop. The methodology consists in obtaining the hydrophilic methanolic extract and the oil, the first comes from the maceration of the seed with methanol and the removal of the lipophilic part with hexane, while the oil is the result of the separation with hexane at the extracts generated by maceration with methanol and acetone sequentially and by extraction with Sohxlet equipment to the final residue with hexane. Thus, metabolites such as free fatty acids, esterified fatty acids, linear triterpenes, steroidal and non-steroidal triterpenes, among others, are identified in the three fractions comprising the oil by GC-MS and 851.49 ± 1.29 mg of -sitosterol and 75.42 ± 1.02 mg of stigmasterol per 100 g of oil. While with the GC-FID and FAME standards, the presence of oleic, palmitic, stearic and (9E) -octadecenoic acids is reported, which make up 30% of the oil. The content of total phenols is 1130.63 ± 11.89 mg EAG / 100 g of dry seed and total flavonoids of 661.23 ± 4.73 mg of quercetin / 100 g of dry seed in the hydrophilic methanolic extract. Through UHPLC-ESI-MS / MS, it is reported that metabolites such as amino acids, organic acids, gallic acid, a wide range of flavonoids, among others, are contained in the same extract and with the use of DPPH reagent it is recorded that it possesses a medium inhibitory concentration (IC50) at 41 μg / ml. Based on the results, it is concluded that this agroindustrial waste has an added value to be explored.
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