Citas bibligráficas
Guevara, M., (2021). Modulación inmune de vitamina D en la actividad funcional de monocitos y macrófagos derivados de monocitos entrenados con BCG [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/9982
Guevara, M., Modulación inmune de vitamina D en la actividad funcional de monocitos y macrófagos derivados de monocitos entrenados con BCG []. PE: Universidad Peruana Cayetano Heredia; 2021. https://hdl.handle.net/20.500.12866/9982
@misc{renati/493858,
title = "Modulación inmune de vitamina D en la actividad funcional de monocitos y macrófagos derivados de monocitos entrenados con BCG",
author = "Guevara Becerra, Martha Cristina Abigail",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2021"
}
Background: Trained immunity, a type of immune memory of the innate immune system, is distinguished by an increased cytokine production upon secondary antigenic stimulation. The BCG vaccine induces trained immunity through epigenetic, metabolic mechanisms and autophagy. Vitamin D induces autophagy, however its role in the immune training of human cells is unknown. Objective: To compare the production of TNF-α and IL-6 cytokines in human monocytes / macrophages trained with BCG or ß-glucan (ßG) with and without 1α,25-dihiydroxyvitamin D3 (VD) supplementation. Methodology: Monocytes isolated from healthy donors underwent an in vitro training protocol with BCG or ßG, and were supplemented with VD (10nM or 100nM) on day 0 or day 6. On day 7, the supernatants were isolated for cytokine analysis (TNF-α and IL-6) by ELISA. Results: The cytokine production of cells supplemented only with VD increased significantly upon restimulation (p <0.05). Although it could not be determined whether VD modulates the production of cytokines in trained cells, an inhibitory trend for ßG and a potentiating trend for BCG was observed. Conclusions: 1α,25-dihydroxyvitamin D3 induces a phenotype of trained immunity and possibly potentiates induction by BCG and inhibits ßG in vitro. The use of vitamin D in trained immunity constitutes a potential therapeutic target for chronic infectious, inflammatory and autoimmune diseases.
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