Bibliographic citations
Inga, R., (2009). Caracterización biológica y acción de inhibidores de una fosfolipasa A2 del veneno de la serpiente peruana Lachesis muta [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/896
Inga, R., Caracterización biológica y acción de inhibidores de una fosfolipasa A2 del veneno de la serpiente peruana Lachesis muta [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2009. https://hdl.handle.net/20.500.12672/896
@misc{renati/485402,
title = "Caracterización biológica y acción de inhibidores de una fosfolipasa A2 del veneno de la serpiente peruana Lachesis muta",
author = "Inga Arellano, Rosalina Rosio",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2009"
}
The Lachesis muta’s envenomations induce a complex physiopathology due to the presence of numerous enzymes group such as Phospholipase A2 (PLA2). For this reason, in the present study have been investigated some biochemistries’ properties of PLA2 and its in vitro and in vivo biologics effect. Initially, the enzyme was purified in two chromatographics steps, CM-Sephadex C-50 followed by Sephadex G-50, obtaining a purification factor of 55.3 times and with a yield of 34.1% and a unique protein band of 18749 Da was obtained by SDS-PAGE analysis under reducing and non-reducing conditions, indicating that PLA2 is a monomeric protein. The protein is thermostable maintaining its total activity until 100ºC per 10 minutes, so, it is a Calcium ion depending protein and the activity was strongly inhibited by glutation, cistein, ESTA and PMSF which reduce until more 50% of PLA2 activity. As far as its biologic activity was obtain that Media Hemolytic Doses (DH50) was 4.35µg and 67.6µg to pure enzyme and crude venom respectively, this indicate that PLA2 is 15,5 times more hemolytic than crude venom. Likewise, had demonstrated that Minimum Miotoxic Doses (MMD) is very low to PLA2 (125. 89 μg/ml) while to crude venom was 144,21 μg/ml. The enzyme’s Minimum Edematic Doses (MED) was 91.5 μg. For another hand, the PLA2 didn´t show any hemorrhagic activity until 30 μg but it showed has anticoagulant activity on citrated plasma when the PLA2 concentration was increasing. Additionally, the inmunodiffusion and inmunoelectrophoresis probes showed PLA2 had inmunogenic reactivity against anti lachesic nonovalent serum (INS-Perú), as well as a partial enzymatic activity and hemolytic neutralization.
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