Bibliographic citations
Corahua, L., (2009). Caracterización molecular de las cepas de Escherichia coli uropatógenas aisladas de pacientes mujeres con infección del tracto urinario del distrito de Comas [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1627
Corahua, L., Caracterización molecular de las cepas de Escherichia coli uropatógenas aisladas de pacientes mujeres con infección del tracto urinario del distrito de Comas [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2009. https://hdl.handle.net/20.500.12672/1627
@misc{renati/484261,
title = "Caracterización molecular de las cepas de Escherichia coli uropatógenas aisladas de pacientes mujeres con infección del tracto urinario del distrito de Comas",
author = "Corahua Ventura, Liz Noemí",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2009"
}
The aim of this study was characterize strains of uropathogenic Escherichia coli (UPEC) isolated from woman patient with urinary tract infection (UTI) coming from the health centre: “Policlínico Parroquial Nuestra Señora de la Paz” located in Comas, during the period August 2006 – August 2007. Sixty UPEC isolates were recollected and their phenotypic features were verified in agar Mac Conkey and others biochemical tests. Fifty-two in addition so determine their antimicrobials susceptibility to antibiotics of first line: amoxicillin / clavulanic acid, nalidixic acid, ciprofloxacine, norfloxacine, trimethopim/ sulfamethoxazole, nitrifurantoin and cefuroxime. It was found an increasing resistance with the following antimicrobials: trimethopim/ sulfamethoxazole (60%), ciprofloxacin (63%), amoxicillin / clavulanic acid (75%). On the other hand, molecular features and plasmid profile of 52 strains was determined by processing the samples with alkaline method and visualizing them in agarose gels with the purpose of associate to resistance antimicrobials profile from the data analyses, it was found five groups according to the number and size to the plasmid, the most representative group was B with 25% of all strains. Finally, it was determined the presence of gene pap, that encode the P fimbria, virulence factor associated with pyelonephritis, by using polymerase chain reaction with specific primers, 61.5% of the total population were positive for this gen.
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