Bibliographic citations
Garcia, M., (2015). Relación de la resistencia antimicrobiana con la presencia de plásmidos en cepas de Acinetobacter baumannii aisladas de pacientes internados del Hospital Nacional Guillermo Almenara Irigoyen- Lima 2012 [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/4394
Garcia, M., Relación de la resistencia antimicrobiana con la presencia de plásmidos en cepas de Acinetobacter baumannii aisladas de pacientes internados del Hospital Nacional Guillermo Almenara Irigoyen- Lima 2012 [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2015. https://hdl.handle.net/20.500.12672/4394
@misc{renati/483985,
title = "Relación de la resistencia antimicrobiana con la presencia de plásmidos en cepas de Acinetobacter baumannii aisladas de pacientes internados del Hospital Nacional Guillermo Almenara Irigoyen- Lima 2012",
author = "Garcia Rivera, Myriam Del Carmen",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2015"
}
---- Acinetobacter baumannii is an aerobic, Gram-negative coccobacillus and is considered an emerging nosocomial pathogen that has developed resistance to multiple drugs. The present study aimed to determine the antimicrobial resistance in Acinetobacter baumannii strains isolated from patients hospitalized in the Guillermo Almenara Irigoyen National Hospital and its relationship with the presence of plasmids, and determine the prevalence of this species in that hospital according to the inpatient service , sample type, age, sex and comorbidity, mortality and seasonality factors. 40 A. baumannii strains of clinical origin, isolated from samples of hospitalized patients at the Guillermo Almenara Irigoyen hospital during January and December 2012, were identified. Strains were identified by the MicroScan Automated System and by the conventional method. MacConkey Agar and Leeds Acinetobacter Agar were used, incubating at 37 and 44ºC, respectively. Gram-negative coccobacillus were observed in the Gram stain. Biochemical test including TSI, citrate and gelatin were performed, in addition to the oxidase and catalase tests. Antimicrobial susceptibility testing was performed using the Micro Scan automated system and the disk diffusion method. 100% of the strains showed resistance to third-generation cephalosporin, meropenem, imipenem, ciprofloxacin, ticar / clavulanic acid. 93.5% were resistant to cefepime and trimethoprim-sulfamethoxazole, 95% to levofloxacin, 90% to amikacin, 45% to gentamicin, 37.5% to tobramycin and 30% to tetracycline. 12 resistance antibiotypes were recorded, with more frequency in the intensive care unit service. The plasmid profile of the studied strains was determined, obtaining 31 profiles according to the number and size of the bands, which ranged from about 1,240– 55,459 bp; these were then associated with the resistance patterns and the isolation origin of the strain. Furthermore, strain curing was performed with ethidium bromide at a concentration of 300 µg/ml. Strains that lost resistance were interpreted as strains carrying plasmidic resistance to certain antibiotics. Moreover, a prevalence of 7.42% of total isolated Gram-negative bacteria was determined, presenting a larger number of cases in the summer and winter, with the same proportion for both sexes; the average age of infected patients was 62.314 ± 19.745. A. baumannii was mainly isolated from respiratory samples, followed by blood cultures and biological fluids samples from the UCI service, Internal Medicine 1 and General Surgery 5. The infections were associated to patients with immunosuppression state caused by surgical procedures and underlying diseases such as cancer, chronic liver failure, kidney failure and EPOC. The mortality rate associated with infection was 56.4%. Keywords: Acinetobacter baumannii, nosocomial infection, antimicrobial resistance plasmids, plasmids healing, hospital prevalence.
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