Bibliographic citations
Gonzales, C., (2019). Efecto inhibitorio in vitro del aceite de semilla de la Persea americana (palta) sobre Pseudomonas aeruginosa [Tesis, Universidad Privada Antenor Orrego - UPAO]. https://hdl.handle.net/20.500.12759/5721
Gonzales, C., Efecto inhibitorio in vitro del aceite de semilla de la Persea americana (palta) sobre Pseudomonas aeruginosa [Tesis]. : Universidad Privada Antenor Orrego - UPAO; 2019. https://hdl.handle.net/20.500.12759/5721
@misc{renati/380078,
title = "Efecto inhibitorio in vitro del aceite de semilla de la Persea americana (palta) sobre Pseudomonas aeruginosa",
author = "Gonzales Quispe, Carlos Antonio",
publisher = "Universidad Privada Antenor Orrego - UPAO",
year = "2019"
}
Pseudomonas aeruginosa is a gram-negative bacterium, an opportunistic pathogen in humans with great adaptability, dissemination, persistence and resistance to drugs, which is involved in infectious processes and of greater clinical relevance in hospitals. Persea americana (Avocado) has defensive mechanisms against some bacteria and the antimicrobial activity may reside in its different phytochemical compounds. The objective of the study was to evaluate the in vitro inhibitory effect of the seed oil of the Persea americana (Avocado) on Pseudomonas aeruginosa. The inhibitory effect in vitro was established by the Kirby-Bauer sensitivity test. Ten repetitions were performed per treatment (Persea americana seed oil at different concentrations [25%, 50%, 75% and 100%] and control groups (positive [Ciprofloxacin] and negative [Tween]). Pseudomonas aeruginosa strains. They were plated with Mueller-Hinton agar culture media, the discs were installed at different concentrations and control groups, then incubated at 37 ° C for 24 hours and finished by measuring the halos with a millimeter ruler. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) of the seed oil of Persea americana, was determined by macrodilution in broth, the inoculum of Pseudomonas aeruginosa was added to each test tube with the different concentrations and control groups; then, it was incubated for 24 hours at 37 ° C, after turbidity was observed and subsequently a subculture of each tube was plated for observation of bacterial growth. As a result, the average for each concentration in inhibition halos are: 26.6 mm, 28.0 mm, 32.4 mm and 37.6 mm, corresponding to the concentrations of 25%, 50%, 75% and 100%. The statistical analysis of the data evidenced highly significant statistical inequality between the different groups at different concentrations and control groups (ANOVA: p <0.05). The MIC was 706.5 mg/mL and the CMB was 930 mg/ mL.
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