Citas bibligráficas
Callata, R., (2014). Degradación del fenol presente en aguas residuales de aeronaves mediante Pseudomonas aeruginosa [Tesis, Universidad Nacional de Ingeniería]. http://hdl.handle.net/20.500.14076/3808
Callata, R., Degradación del fenol presente en aguas residuales de aeronaves mediante Pseudomonas aeruginosa [Tesis]. : Universidad Nacional de Ingeniería; 2014. http://hdl.handle.net/20.500.14076/3808
@mastersthesis{renati/258591,
title = "Degradación del fenol presente en aguas residuales de aeronaves mediante Pseudomonas aeruginosa",
author = "Callata Chura, Rose Adeline",
publisher = "Universidad Nacional de Ingeniería",
year = "2014"
}
The present research treats on the degradation of phenol by means of the microorganism Pseudomonas aeruginosa, which has been made in the Microbiology laboratory facilities of the Universidad Nacional de Ingeniería, Lima Perú. Experiments has been done in three steps, the first consisted in the isolation of the microbial strain from water sample from UNIPETROABC-Talara (lot 9, well 7344), soil and water from Pirín-D-Puno, and hydrocarbon sample that was saved (before sealing the well). Cultivation was done in order to obtain the great quantity possible of strains to be studied and characterize them by means of biochemical tests. Te second step consisted in a period of adaptation of the selected strain to be used in the experimental assays. The third step was the use of the Pseudomonas aeruginosa strain, itself in the process of biodegradation of phenol content in aqueous solutions and samples of wastewater from aircraft toilets. Study the influence of the environmental factors has been possible, through an experimental design for the degradation of the phenol in aqueous solutions, in a range of concentration (30 - 70 mg/L phenol), temperature (16 - 28 °C) and matrix (37 - 55 units of calcium alginate beads). In the Pareto diagram obtained the factorial study 2 indicated us that the temperature is a variable of great influence, followed by the matrix and finally the initial concentration of phenol, whit values of 22.316, 11.776 y 5.507, respectively, with the following equation modeling: Yest =43.150 + 8.425 * Matriz + 15.965 * Temperature - 3.940 * []0Fenol + 2.970 * Matriz * Temperature - 2.025 * Matriz * []0 Fenol + 4.255 * Temperature * []oFenol. The optimization of statistical experimental design, was developed through the response surface methodology and central composite design (20 experimental trials), parameters were obtained more optimum: temperature 33 °C, matrix: 62 units of calcium alginate beads, initial concentration of phenol 49 mg/L, removal efficiency of phenol 73.78 %, in a volume of 300 ml, constant aeration of 0.046 vvm, with the following equation: Y = 51.628 + 8.785 * Matriz + 16.202 * Temperature - 3.869 * []o Fenol - 4.051 * Matriz * Matriz - 5.964 * Temperature * Temperature + 1.439 * []o Fenol * []o Fenol + 2.970 * Matriz * Temperature - 2.025 * Matriz * []o Fenol + 4.255 * Temperature * []o Fenol. Analyzing both equation confirms that the temperature is a fundamental parameter in the degradation of phenol. The equilibrium adsorption-biodegradation has been analyzed by models of isotherms, showing that Freundlich model fits better that Langmuir isotherm, with values: KF = 0.328 Umg, 1/n = 0.7158, R = 0.9916; KL =0.020, R2 = 0.809, respectively. In kinetic studies for concentrations of C¡= 10.526 mg/L phenol (original concentration) and 20 mg/L phenol (adjusted concentration) present in aircraft toilet wasterwater, the best model that describes the rate of phenol degradation is the first order kinetics with the following values; K1 = 0.2441 and 0.1167 h-1; R2 =0.9694 and 3.3574 h-1; R2 = 0.9614 and 0.9467, respectively; kinetic model of second order: K2 = 0.1325 and 0.0291 h-1; R2 = 0.9588 and 0.9513, respectively. The adsorption-biodegradation kinetic for concentration of C¡= 10.526 and 20 mg/L phenol, also shows that the pseudo second order model is that best describe it than pseudo first order model and mass transfer model, with the following parameters: K2 = 0.245 and 0.021 (g/mg-h), R2 = 20.985 and 0.924; K1 = 1.8353 and 0.8405 h-1, = 0.9624 an 0.9178; Ko = 0.4157 and 0.3275, R2 = O.783 and 0.864, respectively, for working conditions: temperature 22-28 °C, contact time of 2.75 and 4.5 hours, respectively, matrix 20.13 g (238 calcium alginate beads e/u of diameter 0.369 cm), volume 3L with constant aeration of 0.018 wm. Key words: Batch, adsorption-biodegradation kinetics, degradation of phenol, central composite design, adsorption isotherm, alginate immobilization, response surface methodology, Pseudomonas aeruginosa, biochemical tests, aqueous solution, reaction rates.
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