Bibliographic citations
Mamani, G., (2015). Métodos de selección espermática y tasa de fecundación in vitro en ovocitos de alpaca (Vicugna pacos) [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/1830
Mamani, G., Métodos de selección espermática y tasa de fecundación in vitro en ovocitos de alpaca (Vicugna pacos) [Tesis]. : Universidad Nacional Agraria La Molina; 2015. https://hdl.handle.net/20.500.12996/1830
@mastersthesis{renati/244428,
title = "Métodos de selección espermática y tasa de fecundación in vitro en ovocitos de alpaca (Vicugna pacos)",
author = "Mamani Mango, Guiulfo Duriel",
publisher = "Universidad Nacional Agraria La Molina",
year = "2015"
}
The male alpacas reproductive efficiency is limited by the small size testicular and long period of ejaculation. Our objective is to evaluate the effect of the methods of sperm selection swim-up and washing on the rate of in vitro fertilization of oocytes alpacas. Spermatozoa were obtained by slicing bodies and tails epididymal alpaca slaughtered (n = 7), frozen in liquid nitrogen and after thawing progressive motility (25 ± 5.96%), vitality (32 ± 5.57%) and evaluated sperm concentration (66.5 ± 24.4 x 106 sperm mL-1), once selected by swim-up was obtained 6.78 ± 18%, 22% ± 8.58 and 106 ± 13.5 x 11.0 x 106 mL-1 motility, vitality and sperm concentration respectively , versus 23% ± 4.83, 29% ± 8.30 and 106 ± 52.0 x 28.3 x 106 mL-1 motility, vitality and sperm concentration, respectively, obtained by washing; both showed similar progressive motility and vitality (p> 0.05), but the washing method provided greater sperm concentration relative to swimup. 335 ovaries of alpacas slaughtered, 839 cumulus-oocyte complex (COC) Grade I and II, were used in 13 sessions of in vitro fertilization. COC were matured in maturation medium (27 hours with 5% CO2 and 38.5 ° C in 50 ul drops of 10 COC / drop), partially denuded by gentle pipetting and placed in 45 ul of fertilization medium; then exposing them to a final concentration of 397 and 484 x 103 motility sperm mL-1 for washing and swim-up respectively (without capacitating agent), co-incubated for 72 hours and evaluated only rate segmentation. 35% (147/418) of segmentation was observed swim-up versus 37% (155/421) for washing, with no significant difference (p> 0.05), ie, both methods can be used in processes IVF without affecting fertilization rate.
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