Bibliographic citations
Cauti, M., (2021). Evaluación de medios de cultivo modificados para el desarrollo de un kit microbiológico basado en MODS de Mycobacterium tuberculosis usando un modelo de crecimiento de Mycobacterium smegmatis [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/9663
Cauti, M., Evaluación de medios de cultivo modificados para el desarrollo de un kit microbiológico basado en MODS de Mycobacterium tuberculosis usando un modelo de crecimiento de Mycobacterium smegmatis []. PE: Universidad Peruana Cayetano Heredia; 2021. https://hdl.handle.net/20.500.12866/9663
@misc{renati/1280188,
title = "Evaluación de medios de cultivo modificados para el desarrollo de un kit microbiológico basado en MODS de Mycobacterium tuberculosis usando un modelo de crecimiento de Mycobacterium smegmatis",
author = "Cauti Mendoza, Maria de Grecia",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2021"
}
The Microscopic Observation Drug Susceptibility (MODS) method has provided new opportunities for the rapid, sensitive, specific, and inexpensive detection of Mycobacterium tuberculosis. However, few laboratories have been able to implement this method, mainly due to the complexity of its preparation. To respond to this situation, the industry developed a MODS Kit with ready-to-use liquid reagents, without any success and withdrawing from the market due to low demand. One of the reasons, possibly, is the high cost required to maintain the cold chain for the conservation of the properties of the liquid medium, this includes the cost of the equipment and its maintenance. Therefore, it is desired to develop a prototype kit that includes dehydrated media to be reconstituted at the time of use and does not require refrigerated storage. In this study, the growth of M. smegmatis, as a model of M. tuberculosis, was evaluated in the liquid medium Middlebrook 7H9-OADC (7H9-OADC) in two presentations: lyophilized and mixed, both sterilized with gamma radiation and PANTA. The growth of the mycobacterium in these two media was compared to the growth of the same in the conventional medium MODS 7H9-OADC (conventional MODS). The lyophilized medium was prepared from the sterilized and lyophilized 7H9-OADC medium; The mixed medium was made by mixing the 7H9 base medium with albumin, dextrose and catalase in their natural state (7H9-ADC). Both dehydrated media were efficiently sterilized with gamma radiation and the PANTA reagent. The PANTA reagent restricted the growth of M. smegmatis, while exposure to gamma radiation showed no adverse effect on the growth of M. smegmatis. Candidate media for the implementation of the MODS kit prototype for the diagnosis of M. tuberculosis are lyophilized medium, lyophilized with gamma radiation and mixed with gamma radiation.
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