Bibliographic citations
Valencia, E., (2012). Patrones de recurrencia y resistencia asociadas a la variabilidad genética de Plasmodium vivax durante la malaria asintomática en la localidad de Mazán-Iquitos [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/3491
Valencia, E., Patrones de recurrencia y resistencia asociadas a la variabilidad genética de Plasmodium vivax durante la malaria asintomática en la localidad de Mazán-Iquitos [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2012. https://hdl.handle.net/20.500.12672/3491
@misc{renati/1224633,
title = "Patrones de recurrencia y resistencia asociadas a la variabilidad genética de Plasmodium vivax durante la malaria asintomática en la localidad de Mazán-Iquitos",
author = "Valencia Ayala, Edward",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2012"
}
--- Plasmodium vivax etiologic agent of malaria has a large genetic variability during recurrent episodes of the disease. This recurrence is reported as low prevalence associated with asymptomatic malaria. Also recurrent episodes (reinfection or relapse) can often be mistaken for drug resistance as chloroquine. Therefore the main objective of this study was to correlate the patterns of recurrence and resistance to the genetic variability of P. vivax. In this study, we evaluated the sequential samples of individuals from an endemic region of Peru (Mazán-Iquitos), previously diagnosed with malaria microscopy during active follows and subjected to a standard treatment regimen with chloroquine. Genotyping based on the pvmsp3-α gene, using Nested PCR and enzymatic digestion, identified high genetic variability of P. vivax, from which were identified recurrence patterns established as relapse, from latent stages or homologous hypnozoites (with identical haplotypes) and reinfections (with different haplotypes). The time ranges allow more accurate identification, with higher frequency of relapses by homologous hypnozoites before 90 days post-first evaluation and higher frequencies of reinfection after this period. Also recurrences in the first period of time, for different haplotypes may also be due to heterologous hypnozoites. Complementing the study, the sequencing analysis of the gene pvmdr1, identified SNPs, encoding nonsynonymous mutations related to resistance to chloroquine. These SNPs, through U-Melt software (in sílico analysis), showed variations in the melting temperatures. Finally the results of relative cuantification with Real Time qPCR no showed significant differences in copy number of the pvmdr1 gene.} Keywords: Chloroquine, Genotyping, Haplotypes, Hipnozoite, Recurrence, Asymptomatic Malaria, Variability.
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