Bibliographic citations
Merino, C., (2006). Divergencia de loci microsatélites entre papas silvestres y cultivadas (familia Solanaceae, género Solanum, sección Petota) [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/816
Merino, C., Divergencia de loci microsatélites entre papas silvestres y cultivadas (familia Solanaceae, género Solanum, sección Petota) [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2006. https://hdl.handle.net/20.500.12672/816
@misc{renati/1214161,
title = "Divergencia de loci microsatélites entre papas silvestres y cultivadas (familia Solanaceae, género Solanum, sección Petota)",
author = "Merino Méndez, Carlos Gonzalo",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2006"
}
--- A kit of 18 microsatellite markers, also referred to as simple sequence repeats (SSR), covering all 12 chromosomes of the potato was previously developed for genotyping the cultivated potato. We have assessed its utility on a sample of 50 wild potato species and 2 other species of the same genus at varying phylogenetic distances from its source, the cultivated potato Solanum tuberosum. The SSR markers produced amplicons in 27% to 100% of the 52 species. A sample of them was sequenced. Although repeat motifs were present in most of the amplicons sequenced, a high degree of single nucleotide polymorphism (SNP) and insertion / deletion events (indel) was observed between the repeat motifs and the primer sequence. These results indicate that: firstly, the 18 microsatellite loci produce amplicons in homologous loci; and secondly, homoplasy will be relatively frequent and hence reduce largely the utility of the cultivated potato SSR kit for phylogenetic studies in wild potato species distant from the cultivated potato germplasm base. Despite this, genetic distance analyses using Jaccard similarity index and Neighbor Joining clustering method produced trees roughly matching hypotheses of cladistic relationships based on recent molecular phylogenies, but not on prior series relationships.
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