Citas bibligráficas
De, J., (2021). Caracterización molecular de secuencias asociadas a virus provenientes de Arracacha ( Arracacia xanthorrhiza Bancroft) y su detección en Papa [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4643
De, J., Caracterización molecular de secuencias asociadas a virus provenientes de Arracacha ( Arracacia xanthorrhiza Bancroft) y su detección en Papa []. PE: Universidad Nacional Agraria La Molina; 2021. https://hdl.handle.net/20.500.12996/4643
@mastersthesis{renati/1116834,
title = "Caracterización molecular de secuencias asociadas a virus provenientes de Arracacha ( Arracacia xanthorrhiza Bancroft) y su detección en Papa",
author = "De Souza Pacheco, Joao Adhemir",
publisher = "Universidad Nacional Agraria La Molina",
year = "2021"
}
Arracacha (Arracacia xanthorrhiza Bancroft) is a plant grown in several countries of South America. The commercial product, which is the storage root, has multiple nutritional properties; its fine starch makes it easily digestible and has high levels of calcium and vitamin A. Due to there are very few studies in Andean roots and tubers, the objectives of this thesis are: characterize the sequences associated with viruses detected in arracacha samples by deep sequencing, detect those virus-associated sequences in both arracacha and potato, and evaluate designed primers for use in the rapid detection of the virus-associated sequences. Evaluations were performed on host plants and the viruses detected using the deep sequencing technology were molecularly characterized. Potato black ringspot virus (PBRSV) nepovirus was detected from an in vitro conserved arracacha plant, confirming the presence of the virus not only by nucleotide and amino acid identity in key ORFs, but also by DAS-ELISA with commercial antibodies to Tobacco ringspot virus and PBRSV. A lyophilized sample infected with a potyvirus called Arracacha potyvirus 1 (AP-1) was also screened. The virus was sequenced by deep sequencing and two virus isolates were obtained in that sample, which sequences indicated that they correspond to Arracacha mottle virus, previously detected in Brazil. In the same way, two arracacha samples showing symptoms were evaluated by deep sequencing, obtaining two new sequences related to crinivirus and vitivirus. Based on the sequences obtained, primers were designed to evaluate the accessions of arracacha and potato plants collected in farmers' fields in Peru and Colombia. These new sequences were detected in some samples. Attempts of mechanical transmission and by possible insect vectors were negative. However, the genomic organization found in the sequences indicates that they may be new viruses capable of infecting arracacha and potato.
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons