Citas bibligráficas
Soto, J., (2021). Taxonomía fenotípica y molecular de bacterias aisladas de nódulos de Clitoria brachystegia Benth., una leguminosa en peligro de extinción [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4870
Soto, J., Taxonomía fenotípica y molecular de bacterias aisladas de nódulos de Clitoria brachystegia Benth., una leguminosa en peligro de extinción []. PE: Universidad Nacional Agraria La Molina; 2021. https://hdl.handle.net/20.500.12996/4870
@phdthesis{renati/1115222,
title = "Taxonomía fenotípica y molecular de bacterias aisladas de nódulos de Clitoria brachystegia Benth., una leguminosa en peligro de extinción",
author = "Soto Valenzuela, Javier Oswaldo",
publisher = "Universidad Nacional Agraria La Molina",
year = "2021"
}
In this research the morphological, biochemical and molecular characteristics of the microorganisms isolated from the nodules of a legume endemic to the tropical dry forest are described. Morphobiochemical evaluations establish agreement with the phenotypic descriptions of rhizobia. BOX-PCR molecular techniques and amplification of the 16S and recA genes, as well as ultramicroscopy, confirm the presence of symbiont and companion Proteobacteria. Symbiotic specificity between Clitoria brachystegia Benth is reported here for the first time. and its host rhizobia. Forty-seven strains of Rhizobium, Bradyrhizobium and Burkholderia were isolated from nodulated roots collected in five locations with soils ranging from acidic (pH 4,28) to neutral and slightly alkaline (7,32), with high average relative humidity (83%), warm temperatures (19 to 22,5 ° C), up to 858 meters above sea level. In addition, phosphate solubilization values, indoleacetic acid production, siderophores, growth at different levels of temperature, pH, salinity, the effect on nodulation and growth in trap plants were obtained; as well as in symbiotic nitrogen fixation values, through the acetylene reduction test (ARA), with ranges significant to the ecosystem, ranging from 0,5 to 54,27 µmol ethylene h-1 g -1 . The objective of this research was to show the typification, taxonomic relationships, diversity and a probable symbiotic specificity of C. brachystegia with its rhizobia, the latter perhaps limited by the particular edaphoclimatic factors of a habitat considered one of the most threatened ecosystems in the world; and, that in the future could be key to the conservation and reforestation of this endangered legume, reported in the socalled Tumbes region shared by Peru and Ecuador.
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