Bibliographic citations
Ruiz, A., (2023). Evaluación del comportamiento en la micropropagación de las especies Cattleya rex y Cattleya violacea mediante el sistema de inmersión temporal [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/5567
Ruiz, A., Evaluación del comportamiento en la micropropagación de las especies Cattleya rex y Cattleya violacea mediante el sistema de inmersión temporal []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2023. http://hdl.handle.net/11458/5567
@phdthesis{renati/1055552,
title = "Evaluación del comportamiento en la micropropagación de las especies Cattleya rex y Cattleya violacea mediante el sistema de inmersión temporal",
author = "Ruiz Rios, Astriht",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2023"
}
Evaluation of the micropropagation performance of Cattleya rex and Cattleya violacea species using the temporary immersion system In this study, the optimal culture medium for the micropropagation of Cattleya rex and Cattleya violacea using a temporary immersion system was determined. The effect of three immersion times and three immersion frequencies on shoot proliferation, the influence of seedling density per temporary immersion system for seedling propagation of both species, the influence of substrate on chlorophyll content in leaves of Cattleya rex and the influence of nitrogen, phosphorus and potassium on the treatments were evaluated. The experiment was carried out in the plant tissue culture laboratory of Corporación G y G Limited Liability Company. Four treatments were used: T0 (3 ml./l of complex B, 7.1 ml/l of bayfolan, 20 g./l of white sugar, 40 g/l of plantain, 100 ml/l of coconut water), T1 (T0 + 1 mg/l of ANA), T2 (T0 + 1 mg/l of 6-BAP) and T3 (T0 + 1 mg/l of ANA + 1 mg/l of 6-BAP), with an immersion time and frequency of 5 minutes and 3 hours, respectively. The effect of three immersion times (5, 10 and 15 minutes), three immersion frequencies (3, 6 and 8 hours) and three densities (20, 40 and 60 seedlings) was evaluated. The best culture medium determined in both species in the temporary immersion system was at a frequency of 3 hours, immersion time of 5 minutes and density of 20 seedlings in 500 ml. of culture medium (3 ml./l of complex B, 7.1 ml/l of bayfolan, 20 g./l of white sugar, 40 g/l of plantain, 100 ml/l of coconut water + 1 mg/l of 6-BAP). The substrate that best influenced the chlorophyll content in Cattleya rex leaves was the T2 treatment (fern + spagnum). Nitrogen, phosphorus and potassium were additional elements for enrichment purposes for explant propagation, with benzylaminopurine influencing shoot formation.
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