Citas bibligráficas
Luna, A., (2022). Optimización de las condiciones de proceso para la obtención de extractos de la hoja de Copaifera officinalis (Copaiba) obtenidos por CO2 supercrítico [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/4906
Luna, A., Optimización de las condiciones de proceso para la obtención de extractos de la hoja de Copaifera officinalis (Copaiba) obtenidos por CO2 supercrítico []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2022. http://hdl.handle.net/11458/4906
@misc{renati/1055484,
title = "Optimización de las condiciones de proceso para la obtención de extractos de la hoja de Copaifera officinalis (Copaiba) obtenidos por CO2 supercrítico",
author = "Luna Arevalo, Anita Valeria",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2022"
}
The present work consists of optimizing the process conditions for obtaining Copaifera officinalis (Copaiba) leaf extracts obtained through supercritical CO2. For this purpose, it was based on characterizing the proximal composition of copaiba leaf, determining the extraction curve and evaluating the influence of temperature and pressure on copaiba leaf extracts for the overall extraction yield, phenolic compound content (TPC) and antioxidant activity by the methods (DPPH and ABTS). The research was carried out through the obtaining of extracts from copaiba leaf using the technique called supercritical plus cosolvent extraction, where ethanol was the cosolvent. Extraction with supercritical carbon dioxide has been widely used as it is considered a green solvent. In the present case, 2 variables are analyzed throughout the investigation: temperature (41, 45, 55, 65 and 69 °C) and pressure (11, 13, 18, 23 and 25 MPa), keeping constant the cosolvent flow rate: 2 mL/min and CO2 flow rate: 0.5 mL/min. The proximate composition of copaiba leaf (lipids 12.06%, crude fiber 18.24%, ash 3.81%, moisture 0.88%, total protein 8.22%, carbohydrates 75.03%). The highest yield (17.73 ± 0.23 %) obtained in the extracts was at a temperature of 65 °C, being the temperature who significantly influenced this analysis. The highest recovery of phenolic compounds (2.604 ± 0.318 mg AGE/gram of raw material) was found at 65 °C, being the temperature also who influenced the optimization of phenols. The highest concentration of antioxidant activity by ABTS (511.591 ± 2.245 μmol TE/gram raw material) was observed in a zone at a temperature of 55 °C and pressures of 11 and 18 MPa. Therefore, the ABTS method applied to the extracts proved to be more efficient in the detection of antioxidant compounds not identifiable with the DPPH method. In conclusion, the results show a positive influence of temperature during the extraction of phenolics and antioxidants, demonstrating that CO2/ethanol extraction is very selective. It is recommended to study other alternative separation techniques and other modifiers in order to evaluate the extraction efficiency.
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