Citas bibligráficas
Garcia, N., (2024). Recuperación de compuestos fenólicos del residuo de naranja (Citrus sinensis) mediante Soxhlet automatizado y determinación de su actividad antioxidante [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/5933
Garcia, N., Recuperación de compuestos fenólicos del residuo de naranja (Citrus sinensis) mediante Soxhlet automatizado y determinación de su actividad antioxidante []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2024. http://hdl.handle.net/11458/5933
@misc{renati/1055377,
title = "Recuperación de compuestos fenólicos del residuo de naranja (Citrus sinensis) mediante Soxhlet automatizado y determinación de su actividad antioxidante",
author = "Garcia Gomez, Noemi",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2024"
}
Each year, the agri-food industry generates tons of by-products and waste. In addition, new technologies for the extraction of bioactive compounds seek high purity extracts and environmentally friendly procedures, as well as the valorization of this biomass generated in the agroindustry for the development of functional foods and pharmaceuticals. For this reason, the present study aimed to determine the percentage of phenolic compounds present in the extract of orange (Citrus sinensis) residue, using the automated soxhlet extraction technique and to determine its antioxidant activity. The raw material presented high carbohydrate content 83.3 g/100 g of sample, fiber 11.2 g/100 g of sample and protein 5.2 g/100 g of sample. For extraction, the factors, solvent/water ratio (50/50, 75/25 and 100/00 %v/v) and extraction time (60 and 120 min) were evaluated. Higher extraction yield was achieved with ethanol at the solvent/water ratio 75/25 (%v/v) at 120 min (51.83±131% yield) than with methanol at solvent/water ratio 100/00 (%v/v) and at 120 minutes (46.26±1.16 % yield). The evaluation of total phenols was carried out by the Folin Ciocalteau method where the highest efficiency with ethanol presented the solvent/water ratio 75/25 (%v/v) at 120 minutes, reporting 5.85±0.20 mgGAE/g ms. In the case of methanol, the solvent/water ratio 50/50 (%v/v) and 75/25 (%v/v) both at 120 minutes were the most efficient, with 5.19±0.19 mgGAE/g ms and 5.62±0.12 mgGAE/g ms, respectively. The ABTS and DPPH methods were used to determine the antioxidant activity. By ABTS using ethanol the solvent/water ratio 50/50 (%v/v) and 75/25 (%v/v) both at 120 minutes were the most efficient, reporting values 39.46±2.83 % inhibition and 38.12±0.74 % inhibition. However, with methanol the treatments did not show significant differences. Similarly, by DPPH, using ethanol, the solvent/water ratio 50/50 (%v/v) and 75/25 (%v/v) both at 120 minutes, presented a higher percentage of inhibition, with 63.50±1.86 % inhibition and 69.02±0.83 % inhibition, respectively. On the other hand, methanol showed no significant difference between treatments. This allows to conclude that both the solvent/water ratio and the extraction time influence the extraction yield, phenolic compounds and antioxidant activity of the orange residue.
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