Citas bibligráficas
Gutierrez, A., (2023). Aplicación de fitohormonas en segmentos nodales para la propagación in vitro de Vanilla pompona Schiede [Universidad Nacional de San Martín]. http://hdl.handle.net/11458/5734
Gutierrez, A., Aplicación de fitohormonas en segmentos nodales para la propagación in vitro de Vanilla pompona Schiede []. PE: Universidad Nacional de San Martín; 2023. http://hdl.handle.net/11458/5734
@mastersthesis{renati/1054943,
title = "Aplicación de fitohormonas en segmentos nodales para la propagación in vitro de Vanilla pompona Schiede",
author = "Gutierrez Ruiz, Astrid Domy",
publisher = "Universidad Nacional de San Martín",
year = "2023"
}
The Vanilla genus is of great potential for the commercialization of its capsules from which the natural vanilla essence is extracted for food and cosmetic use. The species under study does not have a methodology for micropropagation since the interest on it is recent, so the objective of this research was the application of phytohormones in nodal segments for in vitro propagation, testing the protocol of disinfection of nodal segments with NaClO at different concentrations (T1 0.5%, T2 1.0%, and T3 1.5%). Nodal segments from the genetic stock of the Corporación G y G E.I.R.L. nursery were used, which underwent the disinfection process by cleaning with alcohol and placing them in sterile flasks to continue sterilization in the laminar flow chamber. The best result was 1.5% NaClO. To establish the best culture medium, four media were used with M&S as a base (T1, T2, T3, and T4) and as constants the additives of 0.5 mg/L nicotinic acid, 0.4 mg/L thiamine, 20 g/L sucrose, 10 g/L agar (pH between 5.4 and 5.6); with 0.5 ml/L 6-benzylaminopurine at T2, with 1 ml/L 6-benzylaminopurine and 0.5 ml/L naphthalene acetic acid at T3, and with 1.5 ml/L 6-benzylaminopurine and 1 ml/L naphthalene acetic acid at T4. The best result was obtained with the T3 treatment (1 ml/L BAP and 0.5 ml/L ANA), which gave an average of 5.56 shoots.
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