Citas bibligráficas
Martinez, K., (2023). Identificación del virus mosaico de caupí (Vigna ungüiculata L. Walp) con especies indicadoras mediante pruebas de transmisión y propiedades físicas [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/5180
Martinez, K., Identificación del virus mosaico de caupí (Vigna ungüiculata L. Walp) con especies indicadoras mediante pruebas de transmisión y propiedades físicas []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2023. http://hdl.handle.net/11458/5180
@misc{renati/1054429,
title = "Identificación del virus mosaico de caupí (Vigna ungüiculata L. Walp) con especies indicadoras mediante pruebas de transmisión y propiedades físicas",
author = "Martinez Carranza, Keila",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2023"
}
In the San Martin region, there are several diseases affecting Vigna ungüiculata L. known as "cowpea" that show symptoms of mild and strong mosaic, also showing other symptoms, causing low yields, however, the causal agent is unknown. In order to identify the causal agent of cowpea mosaic virus (Vigna ungüiculata L. Walp), the present thesis was carried out at the Plant Health Laboratory of the Faculty of Agricultural Sciences of the National University of San Martin. The transmission tests were carried out on seed evaluated one month after seedling emergence, while mechanical transmission with raw juice on indicator plants and insect vectors was carried out with aphids reared on "Orejon" oregano plants and put to fasting for two hours; physical properties were performed by serial dilution for final dilution point, thermal inactivation point and in vitro longevity, complemented with the DAS-ELIZA serological test. The results show that virus transmission by seeds in cowpea Black eye variety was 1.29% in the nursery and 6.39% in the experimental field of Centro Agroforestal Aucaloma; Symptomatology in indicator plants showed local symptoms in white and marbled panamito bean, huasca huallaguino bean, ashpa bean, Chenopodium quinoa and Amaranthus amaranticolor and systemic symptoms in cowpea Black eye white pod, white cumbaza, bayo ashpa and casma. Transmission by insect vectors (Aphis spp) was of the non-persistent type in Vigna ungüiculata L. Walp, Black eye variety. The results of physical properties performed on Vigna ungüiculata Black eye Variety, final dilution point was 10-3 to 10-4, thermal inactivation point 55 to 60 °C and in vitro infective longevity for 48 hours. In conclusion, the three tests performed are related to the Bean common mosaic virus and the DAS-ELISA serological test confirms that cowpea mosaic virus is present in the San Martin region. Thanks are due to the head of the Plant Health Laboratory of the Faculty of Agrarian Sciences for allowing the research work and to the National Service of Agrarian Health of Peru for providing the service of Virus Serological Identification.
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