Citas bibligráficas
Saavedra, J., (2023). Micropropagación de pitahaya naranja de Churuja (Hylocereus sp.) a partir de fragmentos de cladodios en condiciones in vitro [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/6121
Saavedra, J., Micropropagación de pitahaya naranja de Churuja (Hylocereus sp.) a partir de fragmentos de cladodios en condiciones in vitro []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2023. http://hdl.handle.net/11458/6121
@misc{renati/1054104,
title = "Micropropagación de pitahaya naranja de Churuja (Hylocereus sp.) a partir de fragmentos de cladodios en condiciones in vitro",
author = "Saavedra Garcia, Jose Maximino",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2023"
}
The main objective of this thesis was to micropropagate Churuja orange pitahaya (Hylocereus sp.) from cladode fragments under in vitro conditions. Pitahaya seeds were introduced under in vitro conditions, using disinfectant solutions and MS culture medium. The germinated seedlings were used for the multiplication phase by segmentation of the cladode and subsequent introduction into different culture media with a combination of Benzylaminopurine (BAP) and indole-3-butyric acid (IBA). The shoots formed were separated and induced to root with different concentrations of AIB. Subsequently, the complete regenerated seedlings were taken to the nursery for acclimatization. As a result, 0% contamination and 100% germination were obtained in the seed disinfection phase. 100% of explants with shoot formation and 9.67 shoots on average per explant were obtained with MS culture medium added with 2 mg/L of BAP and 0.1 mg/L of AIB in the multiplication phase. For the rooting phase, a response of 100% of explants with root formation, 3.26 roots per explant and 4.12 cm of root length per explant was obtained using MS culture medium added with 1 mg/L of AIB. Finally, in the case of seedling acclimatization, 100% survival was obtained, seedlings with 16.18 cm length, 9.06 cm diameter, 4.37 cm3 volume, 13.85 cm2 area, 5.2 roots and 7.91 cm root length using the moss and sand substrate in a 2:1 ratio. In conclusion, the study has demonstrated the importance of determining the optimal culture media for the establishment of a micropropagation protocol under in vitro conditions of Churuja orange pitahaya (Hylocereus sp.) from cladode fragments, allowing to obtain a large number of vigorous seedlings.
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