Citas bibligráficas
Santa, J., (2024). Uso de extractos orgánicos en la micropropagación de la orquídea (Cattleya rex) con fines de conservación [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/6098
Santa, J., Uso de extractos orgánicos en la micropropagación de la orquídea (Cattleya rex) con fines de conservación []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2024. http://hdl.handle.net/11458/6098
@misc{renati/1053987,
title = "Uso de extractos orgánicos en la micropropagación de la orquídea (Cattleya rex) con fines de conservación",
author = "Santa Cruz Padilla, Juan Luis",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2024"
}
Use of organic extracts in the micropropagation of the orchid (Cattleya rex) for conservation purposes Different organic sources such as coconut water and fruit extracts present significant concentrations of amino acids, vitamins and organic compounds, capable of acting as growth regulators and being excellent additives for in vitro cultivation of orchids. At the same time, the development of different human activities puts the conservation of orchids such as Cattleya rex in danger of extinction, so alternatives must be sought for the micropropagation of the species for conservation purposes. The study aimed to evaluate the use of organic extracts of pineapple and cocona in the micropropagation of the orchid C. rex for conservation purposes. The population and sample consisted of flasks containing culture media with seeds and plants of C. rex. The research was experimental with a randomized complete block design (RCBD), with four treatments of sodium hypochlorite (NaClO) to evaluate seed disinfection: T0 (0 % NaClO), T1 (0.1 % NaClO), T2 (0.25 % NaClO) and T3 (0.5 % NaClO); and, five treatments to evaluate the development of protocorms, height, number of leaves and roots: T0 (no pineapple and cocona extract), T1 (12.5 g pineapple), T2 (25.0 g pineapple), T3 (12.5 g cocona) and T4 (25.0 g cocona). A protocol was developed and the optimal NaClO treatment for seed disinfection was determined. Five culture media were prepared to evaluate the day of initiation and percentage of protocorms formation at day 30; finally, the same culture media with pineapple and cocona extract were used to evaluate the development of height, number of leaves and number of roots of plants of C. rex plants. Descriptive and inferential statistics were developed for data processing and analysis using the analysis of variance test and Tukey test at 95 % confidence, the statistical analysis was performed in the InfoStat program. It was determined that 0.5 % NaClO favored a greater disinfection of C. rex seeds; likewise, significant differences were determined in the days of initiation and formation of protocorms at day 30, being the culture medium with 12.5 g of pineapple the optimal treatment that favored the initiation of protocorms formation in the least number of days (21.1 days) and the highest percentage of protocorms at day 30 (100.0 %). Finally, there were significant differences in the development of the height and number of leaves of C. rex plants according to treatments, whose optimal culture medium was 12.5 g of pineapple, which allowed determining the greatest average height (19.58 mm) and average number of leaves (14.88 leaves/plant); in addition, the treatments used did not influence the number of roots. It is concluded that the use of organic extracts of pineapple and cocona does favor the micropropagation of Cattleya rex for conservation purposes, promoting the development of height and number of leaves.
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