Bibliographic citations
Quiñones, T., (2022). Actividad antimicrobiana in vitro del aceite esencial de Dalea carthagenensis (Jacq.) J.F.Macbr. “Pata de Perdiz” contra Escherichia coli y Salmonella choleraesuis. [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/19342
Quiñones, T., Actividad antimicrobiana in vitro del aceite esencial de Dalea carthagenensis (Jacq.) J.F.Macbr. “Pata de Perdiz” contra Escherichia coli y Salmonella choleraesuis. []. PE: Universidad Nacional de Trujillo; 2022. https://hdl.handle.net/20.500.14414/19342
@misc{renati/1048356,
title = "Actividad antimicrobiana in vitro del aceite esencial de Dalea carthagenensis (Jacq.) J.F.Macbr. “Pata de Perdiz” contra Escherichia coli y Salmonella choleraesuis.",
author = "Quiñones Morón, Tercila Noemí",
publisher = "Universidad Nacional de Trujillo",
year = "2022"
}
ABSTRACT This investigation was carried out to determine the in vitro antimicrobial activity of the essential oil extracted from Dalea carthagenensis (Jacq.) J.F.Macbr. "Pata de Perdiz" against strains of pathogenic bacteria such as Escherichia coli and Salmonella choleraesuis. The essential oil was obtained from fresh leaves of the plant, through the hydrodistillation method using the Clevenger equipment. The essential oil was characterized by gas chromatography coupled to mass spectrometry (GC-MS). Thirty-six metabolites were identified, the main five were α-Pinene (15.57%), caryophyllene (8.38%), β-Pinene (7.03%), caryophyllene oxide (8.38%), and limonene (5.72%). The antimicrobial activity was determined by microdilution with CTT staining accompanied by the microplate spectrophotometric method. Nine concentrations were used: 2000 μg/ml, 1000 μg/ml, 500 μg/ml, 250 μg/ml, 125 μg/ml, 62.5 μg/ml, 31.25 μg/ml, 15.6 μg/ml, 8 μg/ml, also a positive control (chloramphenicol) and a negative control were used. The results showed that the D.carthagenensis essential oil showed a slight antimicrobial activity against E. coli and Salmonella choleraesuis at concentrations of 2000 μg/ml and 1000 μg/ml. In the other concentrations, D.carthagenensis essential oil did not present antimicrobial activity against any of the two bacteria tested. We concluded that the D.carthagenensis presents a slight reduction in bacterial growth in vitro, compared to the pharmacological control and compared to a zero concentration (medium + bacteria). We recommend test the essential oil on other microorganisms.
This item is licensed under a Creative Commons License