Citas bibligráficas
Ruiz, C., (2024). Especies nativas de Streptomyces del ACP Lomas del Cerro Campana con efecto inhibitorio frente a Staphylococcus aureus y Pseudomonas aeruginosa [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/21159
Ruiz, C., Especies nativas de Streptomyces del ACP Lomas del Cerro Campana con efecto inhibitorio frente a Staphylococcus aureus y Pseudomonas aeruginosa []. PE: Universidad Nacional de Trujillo; 2024. https://hdl.handle.net/20.500.14414/21159
@mastersthesis{renati/1047577,
title = "Especies nativas de Streptomyces del ACP Lomas del Cerro Campana con efecto inhibitorio frente a Staphylococcus aureus y Pseudomonas aeruginosa",
author = "Ruiz Vidaurre, Cynthia Susana",
publisher = "Universidad Nacional de Trujillo",
year = "2024"
}
Actinobacteria are of great importance due to their totalitarian distribution and the production of secondary metabolites of great relevance as antimicrobials. The genus Streptomyces, which in several investigations have described and observed their potentialities, including the secretion of antimicrobial metabolites even under extreme conditions, stands out. This study aimed to isolate native Streptomyces species with higher inhibitory effect against Staphylococcus aureus ATCC43300 and Pseudomonas aeruginosa ATCC27853, from soils of the ACP Lomas del Cerro Campana, Trujillo (Peru), between May and June 2022. For this purpose, 100 g of soil were collected at five points (A1, A2, A3, A4 and A5), homogenized into a complex sample, from which 10 g were taken and serial dilutions (101 -107) were made and surface inoculated on plates with Actinomyces Agar (AA) with Nalidixic Acid (50 ug/ml) and Cycloheximide (50 ug/ml), in triplicate and incubated at 28° C for 13 days. Differential macroscopic and microscopic characteristics of Streptomyces spp. were observed in AA with Nalidixic Acid and Cycloheximide, at 28° C for 13 days. The presumptive cultures of Streptomyces spp. were evaluated for their inhibitory effect against Staphylococcus aureus ATCC43300 and Pseudomonas aeruginosa ATCC27853 by the plate diffusion method; the culture with the highest inhibitory effect was molecularly identified by sequencing the 16s rDNA gene. Three different cultures with presumptive characteristics of the Streptomyces genus (C-01, C-02 and C-03) were obtained, which upon evaluation of their inhibitory effect generated inhibition halos between 8 and 16 mm; highlighting C-01, which generated an inhibitory halo of 16 mm for both bacteria; this culture was molecularly identified as Streptomyces coelicolor. This research concluded in the isolation of the native species of Streptomyces coelicolor with inhibitory effect against Staphylococcus aureus ATCC43300 and Pseudomonas aeruginosa ATCC27853.
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons