Bibliographic citations
Rojas, C., (2021). Influencia del medio de cultivo en la Propagación In Vitro de Espécies de Peperomia de las Regiones de Lambayeque y Cajamarca [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/19301
Rojas, C., Influencia del medio de cultivo en la Propagación In Vitro de Espécies de Peperomia de las Regiones de Lambayeque y Cajamarca []. PE: Universidad Nacional de Trujillo; 2021. https://hdl.handle.net/20.500.14414/19301
@phdthesis{renati/1047104,
title = "Influencia del medio de cultivo en la Propagación In Vitro de Espécies de Peperomia de las Regiones de Lambayeque y Cajamarca",
author = "Rojas Idrogo, Consuelo",
publisher = "Universidad Nacional de Trujillo",
year = "2021"
}
The techniques developed by plant biotechnology, including in vitro plant tissue culture, constitute a fundamental tool that has served to solve multiple problems in agriculture, from the eradication of systemic diseases through the cultivation of meristems, to obtaining of plants from genetically modified cells. Among them, the most accessible and least expensive is micropropagation, applicable to a wide range of species including those of medicinal importance. In this context, the Peperomia species that are members of the flora of the Lambayeque and Cajamarca region, due to their phytochemical and pharmacological potential and environmental impact suffered by their habitats, in the present investigacion the objective was to determine the influence of the culture medium on the in vitro propagation of Peperomia species from two regions that share ecosystems. Eighteen species of Peperomia were used, a high degree of contamination was presented, especially in epiphytic species, oxidation was insurmountable in species from seasonally dry environments. Eight species; P.albovittata, P. peltigera, P. trinervis, P. resediflora, P. rotundata, P. obtusifolia, P. galioides and P.tenella responded positively to the medium that included MS salts and the growth regulators ANA, AIA (0.02, 0.05, 0.1 and 0.5 mg / L) and AG3 (0.02mg / L). Only P. trinervis responded to all formulations including combinations with BAP and KIN cytokinins (0.02 mg / L). Although the number of shoots was relatively low between 1 to 4 on average, it was enough to conclude that in the culture medium constituted by the MS salts supplemented with IAA (0.002 mg / L) and AG3 (0.02 mg / L) it was the Ideal medium for the in vitro propagation of all Peperomia species, remaining for a period of 12 months or more, auguring possibilities of in vitro conservation.
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