Bibliographic citations
Rebaza, C., (2021). Detección de aflatoxina B1 en Granos de kiwicha mediante nanosensores aptaméricos y remediación fotoquímica con rayos ultravioleta [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/21057
Rebaza, C., Detección de aflatoxina B1 en Granos de kiwicha mediante nanosensores aptaméricos y remediación fotoquímica con rayos ultravioleta []. PE: Universidad Nacional de Trujillo; 2021. https://hdl.handle.net/20.500.14414/21057
@mastersthesis{renati/1046877,
title = "Detección de aflatoxina B1 en Granos de kiwicha mediante nanosensores aptaméricos y remediación fotoquímica con rayos ultravioleta",
author = "Rebaza Chavez, Carlos Arturo",
publisher = "Universidad Nacional de Trujillo",
year = "2021"
}
The effectiveness of an aptamer-based nanosensor for detection of aflatoxin B1 (AFB1) in spiked amaranth grains as an alternative method to ELISA was studied in this research project. Furthermore, the photo-degradation activity of ultraviolet irradiation (UV) over spiked grains with AFB1 and Aspergillus spores into a fluidized bed reactor was assessed in order to improve the quality of this agricultural product. AFB1 analysis in amaranth extract through the usage of aptamer-based nanosensors made of gold nanoparticles (gold nanotriangles) allows the detection from 0 to 532 ppb (R2=0,98226). It was noted that a complex matrix like amaranth did not affect the nanosensor ruggedness developed by the QNano+ research group at the Pontificia Universidad Católica del Perú (PUCP). In the near future, this aptamer-functionalized gold nanosensor may be considered as an AFB1 detection kit for amaranth extracts screening. Furthermore, analysis done through ELISA in samples obtained in farmlands at the district of Sanagoran, province of Sanchez Carrion, La Libertad region, did not show AFB1 content (R2=0,9854). Concentrations of AFB1 in spiked amaranth grains were reduced to 33,55% after exposing them to UVA from 30 to 45 minutes. Likewise, the quantity of Aspergillus spores was significantly diminished (99%) after exposing contaminated grains to UVC (R2= 0,9799). Using response surface methodology (RSM) it was found that exposition to UVC for 19,44 minutes and an air flow rate at 1 754,6 L.h-1 was the best treatment. Reduction of AFB1 and spore concentrations in amaranth grains was facilitated by the fluidized bed reactor design which promotes a proper mixing and contact of grains with UVA and UVC irradiation when air flows at high speed (to 3 200 L.h-1) and the sample suspension into the system.
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