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Suárez, J., (2023). Obtención de bioetanol a partir de un consorcio de microalgas amazónicas cultivadas en agua residual [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20194
Suárez, J., Obtención de bioetanol a partir de un consorcio de microalgas amazónicas cultivadas en agua residual []. PE: Universidad Nacional de Trujillo; 2023. https://hdl.handle.net/20.500.14414/20194
@phdthesis{renati/1045487,
title = "Obtención de bioetanol a partir de un consorcio de microalgas amazónicas cultivadas en agua residual",
author = "Suárez Rumiche, Jorge Antonio",
publisher = "Universidad Nacional de Trujillo",
year = "2023"
}
Title: Obtención de bioetanol a partir de un consorcio de microalgas amazónicas cultivadas en agua residual
Authors(s): Suárez Rumiche, Jorge Antonio
Advisor(s): Castillo Valdiviezo, Pascual Ancelmo
Keywords: Micro algas; Biomasa; Azucares reductores; Bioetanol
OCDE field: https://purl.org/pe-repo/ocde/ford#2.04.00
Issue Date: 2023
Institution: Universidad Nacional de Trujillo
Abstract: El bioetanol se extrae de la biomasa de un grupo de microalgas cultivadas en aguas residuales, conocido como consorcio de microalgas, donde se adaptó y cultivo en agua residual domestica por un espacio de treinta días, en reactores cerrado de 7 litros, determinando el perfil microalgal en un espectrofotómetro determinado la densidad óptica a 680 nm, se cosecho por sedimentación – centrifugación, para obtener una biomasa en peso seco (1.05 gr/L). La escisión celular de la biomasa seca, por tratamiento acido a 2, 4 y 6 % de H2SO4, autoclavado a 120 °C durante un tiempo de 30, 60 y 90 min, el contenido de azucares reductores se determinó mediante el método colorimétrico del ácido 3,5-dinitrosalicílico (DNS) a 540 nm, se obtuvieron azucares reductores (1344, 1379 y 1406 mg/L de glucosa) para una hidrolisis del 6% de H2SO4 y tiempo de 30, 60 y 90 minutos, esto demuestra que en tales condiciones se obtuvieron azucares reductores fermentables. La obtención de bioetanol se realizó vía fermentación de los azucares reductores con Saccharomyces cerevisiae para optimizar el tiempo de fermentación se realizaron evaluaciones a 24, 48, 72, 96 y 120 horas. Se obtuvo bioetanol para una conversión de azucares reductores (947 mg/L y 932 mg/L de glucosa) fermentables con levadura de Saccharomyces cerevisiae con un tiempo de fermentación de 48 horas, rendimientos de bioetanol por mg de glucosa fermentada de 93.54 y 92.98 % (mg de etanol/mg de glucosa x 0.511) y los rendimientos de bioetanol por mg de biomasa microalgal de 8.67 y 8.48 %.
Bioethanol is extracted from the biomass of a group of microalgae grown in wastewater, known as the microalgae consortium, where it was adapted and cultivated in domestic wastewater for thirty days, in 7-liter closed reactors, determining the microalgae profile. in a spectrophotometer determined the optical density at 680 nm, it was harvested by sedimentation - centrifugation, to obtain a biomass in dry weight (1.05 gr/L). The cell excision of the dry biomass, by acid treatment at 2, 4 and 6% H2SO4, autoclaved at 120 °C for a time of 30, 60 and 90 min, the content of reducing sugars was determined by the acid colorimetric method. 3,5-dinitrosalicylic (DNS) at 540 nm, reducing sugars (1344, 1379 and 1406 mg/L of glucose) were obtained for a hydrolysis of 6% H2SO4 and time of 30, 60 and 90 minutes, this shows that in Under these conditions, fermentable reducing sugars were obtained. Obtaining bioethanol was carried out via fermentation of reducing sugars with Saccharomyces cerevisiae to optimize fermentation time, evaluations were carried out at 24, 48, 72, 96 and 120 hours. Bioethanol was obtained for a conversion of reducing sugars (947 mg/L and 932 mg/L of glucose) fermentable with Saccharomyces cerevisiae yeast with a fermentation time of 48 hours, bioethanol yields per mg of fermented glucose of 93.54 and 92.98 %. (mg of ethanol/mg of glucose x 0.511) and bioethanol yields per mg of microalgal biomass of 8.67 and 8.48 %.
Bioethanol is extracted from the biomass of a group of microalgae grown in wastewater, known as the microalgae consortium, where it was adapted and cultivated in domestic wastewater for thirty days, in 7-liter closed reactors, determining the microalgae profile. in a spectrophotometer determined the optical density at 680 nm, it was harvested by sedimentation - centrifugation, to obtain a biomass in dry weight (1.05 gr/L). The cell excision of the dry biomass, by acid treatment at 2, 4 and 6% H2SO4, autoclaved at 120 °C for a time of 30, 60 and 90 min, the content of reducing sugars was determined by the acid colorimetric method. 3,5-dinitrosalicylic (DNS) at 540 nm, reducing sugars (1344, 1379 and 1406 mg/L of glucose) were obtained for a hydrolysis of 6% H2SO4 and time of 30, 60 and 90 minutes, this shows that in Under these conditions, fermentable reducing sugars were obtained. Obtaining bioethanol was carried out via fermentation of reducing sugars with Saccharomyces cerevisiae to optimize fermentation time, evaluations were carried out at 24, 48, 72, 96 and 120 hours. Bioethanol was obtained for a conversion of reducing sugars (947 mg/L and 932 mg/L of glucose) fermentable with Saccharomyces cerevisiae yeast with a fermentation time of 48 hours, bioethanol yields per mg of fermented glucose of 93.54 and 92.98 %. (mg of ethanol/mg of glucose x 0.511) and bioethanol yields per mg of microalgal biomass of 8.67 and 8.48 %.
Link to repository: https://hdl.handle.net/20.500.14414/20194
Discipline: Doctorado en Ingeniería Química Ambiental
Grade or title grantor: Universidad Nacional de Trujillo. Escuela de Posgrado
Grade or title: Doctor en Ingeniería Química Ambiental
Juror: Moncada Albitres, Luis Orlando; Ruíz Benites, Segundo Domingo; Castillo Valdiviezo, Pascual Ancelmo
Register date: 13-Dec-2023
This item is licensed under a Creative Commons License