Citas bibligráficas
Yacarini, A., (2022). Epidemiología Molecular de Patotipos de Cepas de Escherichia coli Causantes de Diarrea Infantil de Establecimientos de Salud de la Región Lambayeque, 2018 [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20254
Yacarini, A., Epidemiología Molecular de Patotipos de Cepas de Escherichia coli Causantes de Diarrea Infantil de Establecimientos de Salud de la Región Lambayeque, 2018 []. PE: Universidad Nacional de Trujillo; 2022. https://hdl.handle.net/20.500.14414/20254
@phdthesis{renati/1045356,
title = "Epidemiología Molecular de Patotipos de Cepas de Escherichia coli Causantes de Diarrea Infantil de Establecimientos de Salud de la Región Lambayeque, 2018",
author = "Yacarini Martínez, Antero Enrique",
publisher = "Universidad Nacional de Trujillo",
year = "2022"
}
Acute diarrheal disease (ADD) is classified by the World Health Organization (WHO) as an infectious or non-infectious gastrointestinal inflammation, characterized by a decrease in consistency and an increase in fecal depositions. The present study is descriptive and cross-sectional, whose objective was to determine the molecular epidemiology of pathotypes of Escherichia coli strains that cause childhood diarrhea in health facilities in the Lambayeque region between the months of March to September 2018. The study population was considered to be diarrhogenic E. coli strains, isolated from samples of children under five years of age, from health establishments in the Lambayeque Region, Belén Teaching Hospital, Las Mercedes Regional Teaching Hospital and Lambayeque Regional Hospital, with a total of 75 strains. Analysis of 75 strains of Escherichia coli from diarrheagenic samples was performed by real-time Multiplex PCR using specific primers for genes of the six pathotypes that cause childhood diarrhea: daaD gene (diffusely adherent Escherichia coli - DAEC), aggR gene (Escherichia coli enteroaggregative - EAEC), eaeA gene (Enteropathogenic Escherichia coli - EPEC), stx gene (Shiga toxin-producing Escherichia coli - STEC), ipaH gene (Enteroinvasive Escherichia coli - EIEC) and st gene (Enterotoxigenic Escherichia coli - ETEC). The virulence genes were found in 34.66% (26/75) of the total isolated strains. The Enterotoxigenic E. coli (ETEC) pathotype was the one with the highest frequency, 53.86% (14/26) of the total positive strains. Likewise, it was found that 11.54% of the positive strains (3/26) had more than one pathotype. The detection of specific virulence genes can help to adequately and timely treat an episode of acute childhood diarrhoea.
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