Citas bibligráficas
Alquízar, O., (2023). Eficacia diagnóstica de la amplificación genética por reacción en cadena de polimerasa y del cultivo de Mycobacterium tuberculosis en pleura [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20484
Alquízar, O., Eficacia diagnóstica de la amplificación genética por reacción en cadena de polimerasa y del cultivo de Mycobacterium tuberculosis en pleura []. PE: Universidad Nacional de Trujillo; 2023. https://hdl.handle.net/20.500.14414/20484
@phdthesis{renati/1045213,
title = "Eficacia diagnóstica de la amplificación genética por reacción en cadena de polimerasa y del cultivo de Mycobacterium tuberculosis en pleura",
author = "Alquízar Horna, Oscar Neri",
publisher = "Universidad Nacional de Trujillo",
year = "2023"
}
To compare the efficacy or accuracy of the polymerase chain reaction gene amplification test (PCR test) with the culture of Mycobacterium tuberculosis performed on pleural tissue obtained by needle biopsy, we studied 39 patients with exudative and lymphocytic pleural effusion. Pleural biopsy with Abrams or Cope's needle was executed and the histopathological study, culture for M. tuberculosis in Ogawa medium and nested, in-house PCR test were performed on the pleural tissue. Other diagnostic tests and a follow-up of up to 6 months were performed to obtain the final diagnosis, based on established criteria. Twenty-five patients met the criteria. TB was diagnosed in twenty patients; cancer in four and connective tissue disease in one. For the PCR test the sensitivity was 85%; the specificity, 20%; the predictive value of the positive test (PPV), 81%; the predictive value of the negative test (NPV), 25% and the efficacy 72%. For culture of M. tuberculosis sensitivity was 45%; specificity, 100%; PPV, 100%; NPV, 31.25% and efficacy 56%. In the comparative analysis the PCR test was more sensitive and less specific than the culture, differences that were statistically significant. There was no difference between the predictive values of both tests. The accuracy of the PCR test was greater; but the difference was not statistically significant. We conclude that the PCR GA test was no more effective than the M. tuberculosis culture.
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