Bibliographic citations
Tejada, K., (2024). Producción y caracterización del pigmento prodigiosina de Serratia marcescens obtenida de la fermentación de residuos de escamas de Tilapia [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/22160
Tejada, K., Producción y caracterización del pigmento prodigiosina de Serratia marcescens obtenida de la fermentación de residuos de escamas de Tilapia []. PE: Universidad Nacional de Trujillo; 2024. https://hdl.handle.net/20.500.14414/22160
@mastersthesis{renati/1045143,
title = "Producción y caracterización del pigmento prodigiosina de Serratia marcescens obtenida de la fermentación de residuos de escamas de Tilapia",
author = "Tejada Montenegro, Karen Catalina",
publisher = "Universidad Nacional de Trujillo",
year = "2024"
}
Prodigiosin, a bacterial pigment with antioxidant and pharmacological properties, has been identified with potential applications in the medical and textile industries. This study seeks to produce prodigiosin from tilapia scale waste using Serratia marcescens, a novelty in the field that not only harnesses agro-industrial waste, but also contributes to a reduced environmental impact in the La Libertad region (Peru). Wastewater was collected from a treatment plant, a bacteria from the Serratia genus was isolated and characterized through morphological, biochemical, and molecular tests, including the sequencing of the 16S rRNA gene. In parallel, Tilapia scales were collected and conditioned, then subjected to alkaline hydrolysis. A factorial experimental design was used which evaluated the influence of two factors: the agitation speed (100-250 rpm) and sodium chloride (0.5-2.5%) based on hydrolyzed Tilapia scales, to optimize cell growth and prodigiosin production. It was monitored for 72 hours at 25 ± 1.0 °C. Analytical measurements included determining bacterial growth, estimating prodigiosin and total protein levels. The production of prodigiosin by QP23 was influenced by the agitation speed and sodium chloride in the medium, reaching a maximum peak of 516.03 units per cell at 72 hours with 150 rpm and 1.5% NaCl. The Tukey analysis confirmed significant differences between the combinations of agitation speed and sodium chloride concentrations.
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