Bibliographic citations
Quiñones, C., (2021). Residuos agroindustriales de alcachofa y espárrago como sustrato para la producción de celulosa bacteriana por Komagataeibacter xylinus [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20220
Quiñones, C., Residuos agroindustriales de alcachofa y espárrago como sustrato para la producción de celulosa bacteriana por Komagataeibacter xylinus []. PE: Universidad Nacional de Trujillo; 2021. https://hdl.handle.net/20.500.14414/20220
@mastersthesis{renati/1044517,
title = "Residuos agroindustriales de alcachofa y espárrago como sustrato para la producción de celulosa bacteriana por Komagataeibacter xylinus",
author = "Quiñones Cerna, Claudio Eduardo",
publisher = "Universidad Nacional de Trujillo",
year = "2021"
}
The present research work was carried out with the objective of obtaining bacterial cellulose by Komagataeibacer xylinus from the hydrolyzed residues of artichoke bracts and asparagus peel. The collected agro-industrial waste was washed, disinfected and dried at 60ºC for 6 h, finally ground and sieved at 0.45 mm. In turn, it was characterized by FTIR and its structural sugars by HPLC. The maximum obtaining of reducing sugars from the hydrolysis of agroindustrial residues was evaluated. The production of bacterial cellulose by Komagataeibacter xylinus was evaluated in culture media with different concentrations of reducing sugars of the hydrolyzed residues as a carbon source in Helstrin-Schrianm base medium (without glucose), for 7, 14 and 21 days at 30 ºC in static conditions. From the hydrolyzed residue of artichoke bracts, a maximum bacterial cellulose volumetric yield of 1.59 g / L was obtained from 12.36 g / L ± 0.81 g / L of reducing sugars for 21 days and a bacterial cellulose production rate maximum of 0.21 ± 0.04 g / Ld from 12.36 g / L ± 0.81 g / L for 7 days. In turn, from the hydrolyzed asparagus peel residue, 1.73 ± 0.32 g / L CB was obtained at 21 days from 13.53 ± 0.36 g / L of reducing sugars at 21 days and a production rate of 0.15 ± 0.05 g / Ld CB at 7 days from 13.53 ± 0.36 g / L of reducing sugars. The bacterial cellulose membranes obtained were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC) and atomic absorption microscopy (AFM). The results obtained confirmed that the residues of artichoke bracts and asparagus peladilla could replace the expensive substrates for the production of bacterial cellulose by Komagataeibacter xylinus with remarkable physical, chemical and morphological properties.
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