Citas bibligráficas
Ortiz, M., (2024). “Efecto de antibióticos sobre un sistema de medición de actividad de ARN polimerasa de Mycobacterium tuberculosis en Escherichia coli recombinante” [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/16467
Ortiz, M., “Efecto de antibióticos sobre un sistema de medición de actividad de ARN polimerasa de Mycobacterium tuberculosis en Escherichia coli recombinante” []. PE: Universidad Peruana Cayetano Heredia; 2024. https://hdl.handle.net/20.500.12866/16467
@misc{renati/1041672,
title = "“Efecto de antibióticos sobre un sistema de medición de actividad de ARN polimerasa de Mycobacterium tuberculosis en Escherichia coli recombinante”",
author = "Ortiz Caceres, Maria Carolina",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2024"
}
Antibiotic resistance in Mycobacterium tuberculosis (Mtb) is a global concern. The efficacy of antibiotics targeting the RNA polymerase protein (ARNP) makes it an appropriate target for the development of novel anti-tuberculosis agents. However, antibiotic screening for Mtb carries additional challenges and risks. Recently, the Laboratorio de Moléculas Individuales at UPCH engineered an E. coli that expresses a green fluorescent protein (GFP), dependent on the Mtb ARNP activity produced within it. This reporter bacterium would allow the screening of new inhibitors of this enzyme, thus reducing health risks, expenses and time. However, possible effects on E. coli could make interpretation of results difficult. Therefore, we set out to evaluate antibiotics with action on E. coli to discern between effects on Mtb ARNP and on the host cell. An antibiotic that affects the viability of E. coli (Cefuroxime) and another that affects its ribosomes (Chloramphenicol) were selected. Their effects on bacterial survival, normalized fluorescence, as well as growth and GFP fluorescence rates were evaluated. In addition, their action was compared with other antibiotics that inhibit ARNP-Mtb activity (Rifaximin and Rifampicin). For all antibiotics, a common trend was observed: a reduction in fluorescence that does not necessarily correlate with a decrease in growth. This observation was supported by a mathematical model that showed a similar result, even for Cefuroxime. However, the fluorescence plots indicated that antibiotics that affect ARNP-Mtb generate a slower and steeper drop in GFP production than those that only affect the host bacterium. Although the current system can be used for screening antibiotics targeting ARNP-Mtb, other antibiotics with different targets of action need to be evaluated.
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