Bibliographic citations
Acosta, L., (2010). Colonización intraluminar testicular de células madres germinales a partir de células madre pluripotentes obtenidas de la masa celular interna en ratones [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/3174
Acosta, L., Colonización intraluminar testicular de células madres germinales a partir de células madre pluripotentes obtenidas de la masa celular interna en ratones [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2010. https://hdl.handle.net/20.500.12672/3174
@misc{sunedu/4342873,
title = "Colonización intraluminar testicular de células madres germinales a partir de células madre pluripotentes obtenidas de la masa celular interna en ratones",
author = "Acosta Campos, Láyonal Germán",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2010"
}
Cellular therapy with embryonic or pluripotent stem cells used by regenerative medicine is a newly implemented protocol. These cells are derived from the blastocyst stage inner cell mass (ICM) and are capable of differentiating into all the body cell lineages. On the other hand, chronic diseases treatment such as cancer requires powerful drugs that sometimes cause unwanted effects. Cyclophosphamide (CP) is an alkylating anticancer drug that, among other things, causes infertility as a side effect. The aim of this research was reverse this negative effect by cell therapy using ICM to differentiate in vivo to germ stem cell (GSC). Swiss Rockefeller albino mice blastocyst stage embryos obtained 90 hours post coitus were used. The MCI was isolated by immunosurgery and then transplanted to the seminiferous tubules of recipient mice C57BL, which were previously germ line decreased drastically with CP (220mg/kg pc). The recipient animals were kept for 35 days in standard animal room conditions, after the animals were sacrificated by cervical dislocation; testes were removed to show the ICM colonization and differentiation by serial histological sections. The evaluation found the intraluminal colonization and presence of minitubules (2%) in the transplant recipients (62.5%). It is shown that the ICM cells have the ability to colonize seminiferous tubules of adult mice of different strains. Key words: Intraluminal Colonization, Inner Cell Mass, Pluripotent Stem Cells, Germ Stem Cells, Minitúbules.
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