Bibliographic citations
Ramos, E., (2002). Maduración Final in vitro e in vivo De Los Ovocitos De oncorhynchus mykiss “Trucha Arco Iris” walbaum, 1792. [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1427
Ramos, E., Maduración Final in vitro e in vivo De Los Ovocitos De oncorhynchus mykiss “Trucha Arco Iris” walbaum, 1792. [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2002. https://hdl.handle.net/20.500.12672/1427
@misc{sunedu/4335138,
title = "Maduración Final in vitro e in vivo De Los Ovocitos De oncorhynchus mykiss “Trucha Arco Iris” walbaum, 1792.",
author = "Ramos Maguiña, Eric Steve",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2002"
}
--- In teleosts, the luteinizing hormone begins the final oocyte maturation previous stage of ovulation. The goal of this thesis was to evaluate the efficiency of the human Chorionic Gonadotropin (hCG), luteinizing hormone analogous, to induce in Vitro and in Vivo final oocyte maturation of Oncorhynchus mykiss “Rainbow trout” come from germinal vesicle migration stage. in Vitro final oocyte maturation, the cultures achieved only into Leibovitz L-15 medium (control) showed a very less quantity of mature oocytes. In problem cultures, the culture media that only had 5IU/ml hCG into Leibovitz medium or with Leibovitz L-15 plus 2% (v/v) Oncorhynchus mykiss serum and 5mg/ml insulin produced totality of mature oocytes at 90 hours but when it was used 10UI/ml hCG with the former media were only needed 48 hours to get the totality of mature oocytes. Although the comparative analysis between the different problem media used it showed both the serum and the insulin are important to reach the germinal vesicle breakdown stage (marker used to evaluate in Vitro oocyte maturation) in this fish. The ovulation by means in Vivo maturation was reached at 103.2 hours with first treatment (two doses: 20 and 80 IU hCG/Kg body weight), while the fishes tried with second treatment (two doses: 30 and 120 IU hCG/Kg body weight) ovulated at 43.8 hours. The control fishes reached the maturation at 31 days after their confinement into experimentation pond. These results show the dose-dependent effectiveness for human Chorionic Gonadotropin in the final oocyte maturation of Oncorhynchus mykiss.
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