Bibliographic citations
Meléndez, D., (2015). Efecto del extracto butanólico de Peperomia dolabriformis var. dolabriformis sobre la viabilidad de promastigotos de Leishmania peruviana y el daño de ADN en linfocitos humanos evaluados por el ensayo del cometa [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/19767
Meléndez, D., Efecto del extracto butanólico de Peperomia dolabriformis var. dolabriformis sobre la viabilidad de promastigotos de Leishmania peruviana y el daño de ADN en linfocitos humanos evaluados por el ensayo del cometa []. PE: Universidad Nacional de Trujillo; 2015. https://hdl.handle.net/20.500.14414/19767
@misc{sunedu/3692562,
title = "Efecto del extracto butanólico de Peperomia dolabriformis var. dolabriformis sobre la viabilidad de promastigotos de Leishmania peruviana y el daño de ADN en linfocitos humanos evaluados por el ensayo del cometa",
author = "Meléndez Rodríguez, Deyvi Edwar",
publisher = "Universidad Nacional de Trujillo",
year = "2015"
}
The purpose of this research was to evaluate the effect of butanolic extract from Peperomia dolabriformis var. dolabriformis "congona de zorro" on the viability of Leishmania peruviana promastigotes, and the DNA genotoxic damage on human lymphocytes. The promastigote biomass of Leishmania peruviana from a biphasic medium were cultivated in a liquid medium. The leishmanicidal effect was assayed in a cell density of 106 promastigotes/ml. The negative control T1 was phosphate buffer. Treatment groups T2, T3 and T4 containing 0.009 mg/mL, 0.09 mg/mL and 0.9 mg/mL–butanolic extract were incubated at 27 ° C. The viable promastigotes were counted at 24, 48, 72 and 96 hours. The highest concentration of butanolic extract (T4) with a leishmanicidal effect had an efficiency of 100%. The DNA damage comet assay was evaluated in human peripheral blood lymphocytes that were cultured in PB-max karyotyping medium and incubated for 24 hours at 37°C. Phosphate buffer and 100 uM-hydrogen peroxide were the negative and positive controls. Treatment groups T1, T2 and T3 contained 0.00125%, 0.0025% and 0.01% butanolic extract, respectively. Lymphocytes embedded in a 37°C-LMP agarose were lysed, and subjected to electrophoresis under alkaline conditions at 300 mA, 20 V for 40 minutes. Acridine orange stained slides were examined using a fluorescent microscope, and the DNA damage analyzed by the comet score software. The 0.01%-butanolic extract damaged the DNA with 65.06% in the tail of the comet and 26.49 in the tail moment. It was shown that the butanolic extract of P. dolabriformis var. dolabriformis had leishmanicidal effect on L. peruviana promastigotes, and genotoxic DNA damage in human lymphocytes
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