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Title: Susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente.
Advisor(s): Llenque Díaz, Luis Alberto
Issue Date: 2016
Institution: UNIVERSIDAD NACIONAL DE TRUJILLO
Abstract: Se evaluó la susceptibilidad antimicrobiana de Staphylococcus aureus proveniente de chorizo elaborado artesanalmente, para lo cual se reactivaron los 20 cultivos y prepararon los inóculos bacterianos a una turbidez equivalente al tubo 0.5 del nefelómetro de Mac Farland; luego se sembró, por superficie, con hisopos estériles en agar Muller-Hinton, y se colocaron los discos de antibióticos: vancomicina, rifampicina, ciprofloxacino, doxiciclina, ampicilina, clindamicina, eritromicina y gentamicina en cada placa, se incubaron a 37° C por 18 horas; posteriormente, se midieron los halos de inhibición del crecimiento, en mm y se determinó el grado de susceptibilidad (Sensible, Resistente e Intermedia) teniendo como referencia la tabla de calificación de las normas del NCCLS, 2000. Después de procesar los datos obtenidos, se observó que el 100% de cultivos resultaron sensibles a vancomicina, gentamicina, eritromicina, rifampicina, ciprofloxacino y doxiciclina. Mientras que, el 90% de cultivos fueron sensibles a clindamicina, y el 45% tuvo susceptibilidad intermedia._x000D_ Palabras clave: susceptibilidad antimicrobiana, Staphylococcus aureus, chorizo.
Note: Antimicrobial susceptibility was evaluated of Staphylococcus aureus from sausage hand-crafted, for which 20 crops were reactivated and prepared bacterial turbidity equivalent to a 0.5 tube of inoculants nephelometer Mac Farland evaluated; then seeded by surface with sterile swabs on Muller-Hinton agar and antibiotic discs were placed: vancomycin, rifampin, ciprofloxacin, doxycycline, ampicillin, clindamycin, erythromycin and gentamicin in each plate , incubated at 37 °C for 18 hours ; subsequently, the halos of growth inhibition were measured in millimeters and the degree of susceptibility (sensitive, resistant and medium) was determined with reference to the table qualification standards NCCLS, 2000. After processing the data obtained, it was observed that 100% of cultures were susceptible to vancomycin, gentamicin, erythromycin, rifampicin, ciprofloxacin and doxycycline. While 90% of cultures were sensitive to ampicillin, and 10% were resistant. Likewise, 55% of crops were sensitive to clindamycin, and 45% had intermediate susceptibility. Keywords: antimicrobial susceptibility, Staphylococcus aureus, sausage.
Discipline: Microbiología
Grade or title grantor: Universidad Nacional de Trujillo.Facultad de Ciencias Biólogicas
Grade or title: Biólogo Microbiólogo
Register date:  10



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