Bibliographic citations
Amen, J., (2019). Efecto de dos crioprotectores sobre la viabilidad espermática de zánganos de abejas melíferas (Apis mellifera L.) [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4353
Amen, J., Efecto de dos crioprotectores sobre la viabilidad espermática de zánganos de abejas melíferas (Apis mellifera L.) []. PE: Universidad Nacional Agraria La Molina; 2019. https://hdl.handle.net/20.500.12996/4353
@misc{sunedu/3286611,
title = "Efecto de dos crioprotectores sobre la viabilidad espermática de zánganos de abejas melíferas (Apis mellifera L.)",
author = "Amen Castillo, Joaquín Luis Ernesto",
publisher = "Universidad Nacional Agraria La Molina",
year = "2019"
}
The cryopreservation of drone semen will allow us to use the genetics of the best individuals in the long term. To this end, the effect of the cryoprotectors Dimethylsulfoxide (DMSO) and glycerin on the sperm viability of honeybee drones was evaluated, using two different degrees of semen dilution in the diluter (1:9 and 1:12). The evaluated semen was collected in 15 glass capillaries, coming from drones captured on their return to the hive, being subsequently diluted in the proportions mentioned for each cryoprotector used, finally obtaining four mixtures for each capillary. The content corresponding to five capillaries was frozen until - 20°C and the content of the other ten, until - 40°C, to be cryopreserved later. The motility results of diluted fresh semen and postdefrost semen were measured on a subjective scale (1 to 5), being analyzed with a Friedman test; post-defrost viability data were measured in percentage and submitted to ANOVA. Values of volume, semen collection efficiency and concentration were recorded. No significant difference was found in the results of motility and viability of post-thawed semen, using both DMSO and glycerin, in the semen dilution degrees in the 1:9 or 1:12 diluter, at both freezing temperatures. The observed values of motility and post-thaw viability are not considered optimal for the freezing temperature of - 20°C, contrary to what was observed for the freezing temperature of - 40°C, obtaining greater viability observed for the use of DMSO in either of the two degrees of semen dilution in the diluter.
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