Bibliographic citations
Requis, A., (2020). Identificación y control del agente causal de la pudrición húmeda en alcachofa (Cynara scolymus L.) en Chincha Baja [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/4350
Requis, A., Identificación y control del agente causal de la pudrición húmeda en alcachofa (Cynara scolymus L.) en Chincha Baja []. PE: Universidad Nacional Agraria La Molina; 2020. https://hdl.handle.net/20.500.12996/4350
@mastersthesis{sunedu/3190903,
title = "Identificación y control del agente causal de la pudrición húmeda en alcachofa (Cynara scolymus L.) en Chincha Baja",
author = "Requis Quintanilla, Angela Juana",
publisher = "Universidad Nacional Agraria La Molina",
year = "2020"
}
From the lower Chincha area, samples of the artichoke culture were extracted, two somewhat different symptoms; in one of them, wet rot was observed at neck level, in the aerial part there was wilting and lying down of the plant, when making a cut in the neck internally there was presence of soft rot of light green to dark brown color; the other symptom was characterized by showing at the bud level an aqueous necrosis at the edge of the tender leaves and advanced downward towards the central part compromising the formation of the chapter. Five isolates were positive in the pathogenicity test. Through phenotypic tests such as Gram staining, hole formation in Crystal Violet Pectate medium, catalase, oxidation and fermentation and degradation of potato sheets, it was determined that the isolates belong to the Pectobacterium genus. With tests of sensitivity to erytromycin, growth at 37 ° C, growth in 5% NaCl and degradation of substances from sucrose; it was not possible to determine the subspecies of the isolates, because the results obtained did not coincide with the results of other authors. For the molecular identification test specific primers were used for Pectobacterium carotovorum subsp. carotovorum and another for Pectobacterium carotovorum subsp. atroséptica, when performing the PCR with these primers there was no result, so a set of general primers from the 16S RNA region was used, identifying: Pectobacterium carotovorum subsp. brasiliense (neck and bud), Pectobacterium carotovorum subsp. odoriferum (bud), and Pectobacterium carotovorum subsp. actinidae (neck). In the in vitro control test, copper-based products were the best, and in the field for neck and bud rot the best results were obtained with copper gluconate, copper proteinate, calcium proteinate (alone or in combination with the biocontroller) and Trichoderma (strain T22)
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