Bibliographic citations
Rengifo, D., (2008). Marcadores Rapd ligados al sexo de Mauritia flexuosa l.f. “aguaje” (Arecaceae) en el distrito de San Juan Bautista, Loreto-Perú [Tesis, Universidad de la Amazonía Peruana]. http://repositorio.unapiquitos.edu.pe/handle/20.500.12737/5425
Rengifo, D., Marcadores Rapd ligados al sexo de Mauritia flexuosa l.f. “aguaje” (Arecaceae) en el distrito de San Juan Bautista, Loreto-Perú [Tesis]. : Universidad de la Amazonía Peruana; 2008. http://repositorio.unapiquitos.edu.pe/handle/20.500.12737/5425
@misc{sunedu/3118003,
title = "Marcadores Rapd ligados al sexo de Mauritia flexuosa l.f. “aguaje” (Arecaceae) en el distrito de San Juan Bautista, Loreto-Perú",
author = "Rengifo Trigoso, Dixner",
publisher = "Universidad de la Amazonía Peruana",
year = "2008"
}
The present work's objective was to identify RAPD markers linked to sex of “aguaje” Mauritia flexuosa L. f. from DNA mixtures of male and female plants. The sampling area were the community of Quistococha (latitude 03°49'28.66 " S length 73° 19'18.98" W, with 96 msnm) and Santa Clara (latitude 03°46'50.5" S, longitude 73 °18'25.3" W, with 118 msnm); 10 male and 10 female sexually differentiated plants were selected, Collecting leaves, roots and / or pneumatophores and transported to the Research Laboratory of the Biological Sciences School and the Research Laboratory of Natural Antiparasitic Products of the Amazon (LIPNAA) of the National University of the Peruvian Amazon (UNAP), DNA was extracted and mixed by sex , performing RAPD PCR tests with 38 random primers; of Which, 11 primers (E -36, E -37, D1, UNAP1, F1, F5xFa, R5xFma, UNR, PLF, FAR' and EXT5) generated 31 polymorphic amplification products (21 differential bands on female plants and 10 male) with sizes between 182 to 1.552bp; six bands presented better resolution (502bp, 513bp, 610bp, 762bp, 871bp and 975bp) and the F5xFa primer revealed a greater number of differential bands with the female sex. Therefore; the female sex plants are more polymorphic than the male sex and probably can identify sex with RAPD PCR technique early in M. flexuosa.
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