Bibliographic citations
Liceta, M., (2015). Aislamiento y caracterización de Pseudomonas y Bacillus provenientes de la rizósfera de diferentes variedades de quinua (Chenopodium quinoa Willd.) y su uso como potenciales promotoras del crecimiento vegetal [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/2690
Liceta, M., Aislamiento y caracterización de Pseudomonas y Bacillus provenientes de la rizósfera de diferentes variedades de quinua (Chenopodium quinoa Willd.) y su uso como potenciales promotoras del crecimiento vegetal []. PE: Universidad Nacional Agraria La Molina; 2015. https://hdl.handle.net/20.500.12996/2690
@misc{sunedu/3012628,
title = "Aislamiento y caracterización de Pseudomonas y Bacillus provenientes de la rizósfera de diferentes variedades de quinua (Chenopodium quinoa Willd.) y su uso como potenciales promotoras del crecimiento vegetal",
author = "Liceta Llanco, Mónica",
publisher = "Universidad Nacional Agraria La Molina",
year = "2015"
}
Internationally, the demand of native organic products is acquiring greater interest (attention) due to high nutritional values and their active principles. Quinoa is well known because of its high protean value (12-18%) and its great biological diversity. Currently, Peru is the second greatest producer of quinoa worldwide, reaching 114.3 thousand tons until December 2014. In the agricultural sector, the use of plant growth-promoting rhizobacteria (PGPR) is acquiring greater relevance, both as biofertilizers and as phitopatogens controllers. However, there are only few studies addressing their application to quinoa crops. Thus, the present work aims to evaluate the application of bacterial bioinoculants of Pseudomonas, Bacillus and other strains as a biotechnological alternative, increasing the productivity and the nutritional content in quinoa crops. A presumptive isolation of Pseudomonas and Bacillus strains was carried out from the native rizhosphere of quinoa crops under two temperatures: 28 °C and 10 °C. These bacteria have a rhizospheric origin from three varieties of quinoa crops from the region of Puno. Strains were characterized phenotypically and for their ability as PGPR. The production of indol acetic acid (IAA) was evaluated, being Pseudomonas strains the ones that stood out, with values up to 36.23μg/mL of IAA (PS13) and the solubilization of dicalcic and tricalcic phosphates with an efficiency of up to 259% (PS12). The effects of the temperature on the strains’ growth and their PGPR capacity was also evaluated. In vitro assays were developed with the selected strains based on their PGPR traits, which were applied on seeds from Salcedo variety. At this level, the percentage of seeds’ germination, the presence of pubescence at the roots and the dry weight of the seedlings were evaluated. The greenhouse stage assay was carried out in La Molina – Lima, the strains PS43 and PS44 were selected and their emergence, height, volume of the panicle, dry weight and nutritional content of the obtained grains were evaluated. Seedling inoculated with the PS44 strain (Pseudomonas) had better values in terms of height, with an increase of 26.3% compared to the control from the Salcedo variety. In terms of nitrogen content of the grain, better results were achieved with all the inoculated treatments compared to the control, with values up to 38.2% (PS44).
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