Bibliographic citations
Suárez, C., (2015). Análisis de perfiles plasmídicos de escherichia coli y klebsiella pneumoniae productoras de B-Lactamasas de espectro extendido aisladas en urocultivos en el Instituto Nacional de Salud del Niño [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/4292
Suárez, C., Análisis de perfiles plasmídicos de escherichia coli y klebsiella pneumoniae productoras de B-Lactamasas de espectro extendido aisladas en urocultivos en el Instituto Nacional de Salud del Niño [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2015. https://hdl.handle.net/20.500.12672/4292
@misc{sunedu/2881573,
title = "Análisis de perfiles plasmídicos de escherichia coli y klebsiella pneumoniae productoras de B-Lactamasas de espectro extendido aisladas en urocultivos en el Instituto Nacional de Salud del Niño",
author = "Suárez Rojas, Carlos Josemaría",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2015"
}
--- Background: The progressive increase in resistance rates in uropathogenic bacteria and their spread have become in a significant worldwide public health, becoming more important the spread of resistant isolates producing of extended spectrum ß-lactamases, which are useful the molecular characterization of plasmids and other genetic elements of the bacteria to establish the genetic relationship between them, to have relevant epidemiological data that we can suggest the direction of propagation of this type of resistance. Objetive: Determine the plasmid profiles in isolates of Escherichia coli and Klebsiella pneumoniae producing of extended spectrum ß-lactamases recovered from urine cultures in the service of Microbiology of National Institute of Child Health (NICH). Design: Descriptive, observational cross-sectional study. Institution: Biochemestry and Nutrition Investigation Center CIBN-UNMSM; National Institute of Child Health. Participants: Isolates of Escherichia coli and Klebsiella pneumoniae during November and December of 2012 in the service of Microbiology of National Institute of Child Health. Interventions: Extraction of plasmid DNA from E. coli and K. pneumoniae, detection of plasmid profiles by electrophoresis gel of 0.8% agarose and staining of DNA fragments was carried out using ethidium bromide and they were visualized by UV-Trans illumination, the analysis of fingerprint patterns of plasmid profiles obtained using the NTSYSpc 2.1 program and the UPGMA clustering algorithm. Results: Were detected different electrophoretic patternsin the samples, obtained from 1-7 bands per sample and a total of 15 bands of different sizes, which varied from 1.5 kb to more than 20 kb. Considering the patterns of similarity, the isolates analyzed were distributed into different clusters or branches. Conclusion: The determination of plasmid profiles proved to be a sensitive and reliable technique for the analysis of genetic diversity isolates of E. coli and K. pneumoniae from the National Institute of Child Health. Key words: Plasmid profiles, E. coli, K. pneumoniae, extended spectrum ß- lactamases.
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