Flores, L. (2003). Caracterización fenotípica y genotípica de estirpes de Salmonella choleraesuis aisladas de ambientes marinos [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/803
Flores, L. Caracterización fenotípica y genotípica de estirpes de Salmonella choleraesuis aisladas de ambientes marinos [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2003. https://hdl.handle.net/20.500.12672/803
title = "Caracterización fenotípica y genotípica de estirpes de Salmonella choleraesuis aisladas de ambientes marinos",
author = "Flores Aguilar, Lidia Escolástica",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2003"
--- Pathogenic bacteria like Salmonella, natural inhabitants of the intestinal tract of diverse animals included the man, they are commonly present in effluents of mainly domestic drainages that discharge into the sea in direct form or through rivers and canals, ending up constituting part of the contaminate flora of the Lima´s coast and of the foods coming from the same one. Their presence is determined by the biological and physico chemical conditions of the aquatic environment that allow its survival, developing mechanisms of adaptation like to enter to state of “viable but no culturable”, metabolic and genomic changes in answer to adverse environmental conditions. Due to the importance that Salmonella has in the incidence of gastrointestinal illnesses, it becomes necessary their surveillance in the aquatic environment for that which is necessary to develop studies that facilitate the isolation, identification and differentiation of parthogenic strains in natural ecosystems. Seawater samples were taked along the Lima´s coast, during the months of march, april and may of 2000. For the isolation the membrane filtration method was used, pre-enrichment in Buffered Peptoned Water; selective enrichment in Tetrathionate, Selenite Cystine and Rappaport-Vassiliadis broths and selective isolation in XLD, Bismuth Sulfite, BPLS, SS, Hektoen, XLD agars. In the biochemical identification Oxidasa, Catalasa, Indole tests were used and it was cultivated in Urea, TSI, LIA, Citrato, RM-VP media. For the serologic identification grouping polyvalents (A, B, C1, C2, D, E1 and E4) and flagellars sera of Salmonella choleraesuis were used. 203 strains negative oxidase and positive catalase were selected, of which 18 were identified as Salmonella choleraesuis, inside those that 10 strains belonged to C1 serogroup and Salmonella Djugu serotype, and 8 to D serogroup and Salmonella Enteritidis serotype, one with typical biochemistry and 2 atypical strains typing inside the B serogroup of Salmonella choleraesuis, 3 rough strains and 24 with typical biochemistry that they didn't agglutinate with the polyvalent serum used. The profiles of total proteins obtained by means of SDS-PAGE point out differences of the strains inter and intra serovar after statistical analyses. The chromosomal DNA of the identified serotypes was cut with restriction endonucleases and hibridized with a specific probe for the gene rDNA16S marked by chemioluminiscens. They were 4 ribotypes (RB, RC1, RC2 y RD), that corresponded to each serovar of Salmonella choleraesuis.
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