Bibliographic citations
Hurtado, N., (2021). Desarrollo y validación de una metodología analítica para la determinación de diez residuos de antibióticos veterinarios en Oncorhynchus mykiss, utilizando cromatografía líquida de ultra rendimiento acoplada a un detector de masas de triple cuadrupolo [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/9609
Hurtado, N., Desarrollo y validación de una metodología analítica para la determinación de diez residuos de antibióticos veterinarios en Oncorhynchus mykiss, utilizando cromatografía líquida de ultra rendimiento acoplada a un detector de masas de triple cuadrupolo []. PE: Universidad Peruana Cayetano Heredia; 2021. https://hdl.handle.net/20.500.12866/9609
@misc{sunedu/2792890,
title = "Desarrollo y validación de una metodología analítica para la determinación de diez residuos de antibióticos veterinarios en Oncorhynchus mykiss, utilizando cromatografía líquida de ultra rendimiento acoplada a un detector de masas de triple cuadrupolo",
author = "Hurtado Galindo, Nathaly Elizabeth",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2021"
}
Introduction: In Peru, there are surveillance protocols for antibiotic residues in the food sector, but there are areas in continuous growth that do not comply with this same vigilance emphasis, as is the case with aquaculture. Despite having important products for human consumption such as rainbow trout (Oncorhynchus mykiss), its regulatory entity does not have validated and accepted methods to determine antibiotic residues in aquaculture products. The objective of the current study was to develop and validate an analytical methodology for the determination of ten residues of veterinary antibiotics in Oncorhynchus mykiss, using ultra-performance liquid chromatography coupled to a triple quadrupole mass detector. Methodology: A multi-residue method of 10 antibiotics that can identify and quantify their concentration in samples of rainbow trout was developed and validated, using the liquid chromatography technique coupled to Triple Quadrupole Mass Spectrometry (UHPLC-MS/MS). Selectivity, linearity, sensitivity, detection limit, quantification limit, recovery, precision and range parameters were assessed, based on acceptability criteria of the chemical methods validation guide of the Food and Veterinary Medicine Program of the Food and Drug Administration of the United States. The method was additionally applied to samples of rainbow trout purchased from 6 different sales locations in Lima. Results: The method was successfully developed and validated. It met the validation criteria of selectivity, linearity, sensitivity, limit of detection, limit of quantification, recovery, precision and working range described in the reference guidelines. Also, veterinary drugs residues concentration of the trout samples from different local markets were not detectable by the method, except for one of the evaluated markets (Comas) that showed oxytetracycline traces. Conclusions: The reported method is adequate for the detection and quantification of the analytes of interest in aquaculture rainbow trout samples. Its implementation by the national regulatory authorities is recommended to monitor the safety of the products to be sold nationally and internationally.
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