Bibliographic citations
Pickling, E., (2024). Evaluación de la actividad antimicrobiana, citotoxicidad y biocompatibilidad de un cemento endodóntico experimental con la incorporación de menta, muña, propóleo Jalks y tintura de propóleo / [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/15644
Pickling, E., Evaluación de la actividad antimicrobiana, citotoxicidad y biocompatibilidad de un cemento endodóntico experimental con la incorporación de menta, muña, propóleo Jalks y tintura de propóleo / []. PE: Universidad Peruana Cayetano Heredia; 2024. https://hdl.handle.net/20.500.12866/15644
@phdthesis{renati/911373,
title = "Evaluación de la actividad antimicrobiana, citotoxicidad y biocompatibilidad de un cemento endodóntico experimental con la incorporación de menta, muña, propóleo Jalks y tintura de propóleo /",
author = "Pickling Gonzales Posada, Erwin Duilio",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2024"
}
The antimicrobial properties in natural elements have attracted the attention of researchers as a complement to endodontic cement (EC). This work was carried out in four phases. The first phase identified antibacterial effectiveness of Mentha piperita (mint), of Minthostachys mollis (muña) and Própolis (in propolis tincture and propolis Jalks) in Enterococcus Faecalis (E. Faecalis) strains from the American Type Culture Collection (ATCC 2982). The second phase determined the effectiveness incorporated into the CE, both with antibacterial sensitivity testing in two culture media (brain and heart infusion (BHI), brain and heart agar (BHA)), then Gram staining was applied (verified under a microscope). The Mc Farland test homogenized the amount of bacteria in the Petri plate, the halos were evaluated after 24 hours with a Vernier. The third phase determined the cell viability test (CC50 – MTT), the cells incubated in an atmosphere of 5% CO2 with 37°C for 24 hours at 95% humidity. The sample was incubated with MTT until the formation of crystals (formazan), and then the medium was removed with 10 ml of dihydrogenase. The solubilizing solution (isopropanol 100 microliters) until complete solubilization and analysis with Enzyme Linked Immunosorbent Assay (ELISA) (Thermo Fisher Inc, Waltham, Massachusetts, United States). The fourth phase determined the biocompatibility of the CE in an-In-Vivo model of 12 5-month-old male WISTAR-type rats (300g to 400g). They were depilated, disinfected, anesthetized, incised (10mm long by 2mm deep) for samples and sutured. After 30 days, the samples were extracted and processed. The results in the first phase identified the mint with the greatest inhibition (5.975mm). In the second phase, the control CE presented the greatest inhibition (18,050 ± 0.451 mm), followed by the CE with mint (16,498 ± 0.460 mm). In the third phase the EC presented greater viability and in the fourth phase the EC with propolis presented the greatest biocompatibility (4.00 ± 3.00 cells). With the statistical analysis of variance (ANOVA) and Turkey's post-hoc test it can be concluded that the antibacterial effect of muña alone was superior and its biocompatibility was lower in the CE. The CE with mint was better antibacterial and obtained a higher percentage of viability.
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